Vitamin B2 inhibits glutamate release from rat cerebrocortical nerve terminals

Su Jane Wang; Wen Mein Wu; Feili Lo Yang; Guoo Shyng Wang Hsu; Chia Yu Huang

doi:10.1097/WNR.0b013e32830b8afa

Vitamin B2 inhibits glutamate release from rat cerebrocortical nerve terminals

Su Jane Wang, Wen Mein Wu, Feili Lo Yang, Guoo Shyng Wang Hsu, Chia Yu Huang

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

We examined the effect of ribo£avin, vitamin B2, on the release of endogenous glutamate fromnerve terminals puri¢ed fromrat cerebral cortex.The release of glutamate evoked by 4-aminopyridine was inhibited by ribo£avin. Further experiments indicated that ribo£ avin-mediated inhibition of glutamate release (i) results from a reduction of vesicular exocytosis, not from an inhibition of nonvesicular release; (ii) is associated with a decrease in presynaptic Ntype and P/Q-type voltage-dependent Ca2+ channel activity.These ¢ndings are the ¢rst to suggest that, in rat cerebrocortical nerve terminals, ribo£avin suppresses voltage-dependent Ca2+ channel activity and in so doing inhibits evokedglutamate release.This ¢nding may explain the neuroprotective effects of vitamin B2 against neurotoxicity. NeuroReport 19:1335-1338

Original language	English
Pages (from-to)	1335-1338
Number of pages	4
Journal	NeuroReport
Volume	19
Issue number	13
DOIs	https://doi.org/10.1097/WNR.0b013e32830b8afa
State	Published - 27 Aug 2008

Keywords

Cerebral cortex
Glutamate release
Ribofavin
Synaptosomes
Voltage-dependent Ca channels

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10.1097/WNR.0b013e32830b8afa

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title = "Vitamin B2 inhibits glutamate release from rat cerebrocortical nerve terminals",

abstract = "We examined the effect of ribo£avin, vitamin B2, on the release of endogenous glutamate fromnerve terminals puri¢ed fromrat cerebral cortex.The release of glutamate evoked by 4-aminopyridine was inhibited by ribo£avin. Further experiments indicated that ribo£ avin-mediated inhibition of glutamate release (i) results from a reduction of vesicular exocytosis, not from an inhibition of nonvesicular release; (ii) is associated with a decrease in presynaptic Ntype and P/Q-type voltage-dependent Ca2+ channel activity.These ¢ndings are the ¢rst to suggest that, in rat cerebrocortical nerve terminals, ribo£avin suppresses voltage-dependent Ca2+ channel activity and in so doing inhibits evokedglutamate release.This ¢nding may explain the neuroprotective effects of vitamin B2 against neurotoxicity. NeuroReport 19:1335-1338",

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T1 - Vitamin B2 inhibits glutamate release from rat cerebrocortical nerve terminals

AU - Wang, Su Jane

AU - Wu, Wen Mein

AU - Yang, Feili Lo

AU - Wang Hsu, Guoo Shyng

AU - Huang, Chia Yu

PY - 2008/8/27

Y1 - 2008/8/27

N2 - We examined the effect of ribo£avin, vitamin B2, on the release of endogenous glutamate fromnerve terminals puri¢ed fromrat cerebral cortex.The release of glutamate evoked by 4-aminopyridine was inhibited by ribo£avin. Further experiments indicated that ribo£ avin-mediated inhibition of glutamate release (i) results from a reduction of vesicular exocytosis, not from an inhibition of nonvesicular release; (ii) is associated with a decrease in presynaptic Ntype and P/Q-type voltage-dependent Ca2+ channel activity.These ¢ndings are the ¢rst to suggest that, in rat cerebrocortical nerve terminals, ribo£avin suppresses voltage-dependent Ca2+ channel activity and in so doing inhibits evokedglutamate release.This ¢nding may explain the neuroprotective effects of vitamin B2 against neurotoxicity. NeuroReport 19:1335-1338

AB - We examined the effect of ribo£avin, vitamin B2, on the release of endogenous glutamate fromnerve terminals puri¢ed fromrat cerebral cortex.The release of glutamate evoked by 4-aminopyridine was inhibited by ribo£avin. Further experiments indicated that ribo£ avin-mediated inhibition of glutamate release (i) results from a reduction of vesicular exocytosis, not from an inhibition of nonvesicular release; (ii) is associated with a decrease in presynaptic Ntype and P/Q-type voltage-dependent Ca2+ channel activity.These ¢ndings are the ¢rst to suggest that, in rat cerebrocortical nerve terminals, ribo£avin suppresses voltage-dependent Ca2+ channel activity and in so doing inhibits evokedglutamate release.This ¢nding may explain the neuroprotective effects of vitamin B2 against neurotoxicity. NeuroReport 19:1335-1338

KW - Cerebral cortex

KW - Glutamate release

KW - Ribofavin

KW - Synaptosomes

KW - Voltage-dependent Ca channels

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JO - NeuroReport

JF - NeuroReport

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ER -

Vitamin B2 inhibits glutamate release from rat cerebrocortical nerve terminals

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Keywords

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