TY - JOUR
T1 - Visible light regulates neurite outgrowth of nerve cells
AU - Higuchi, Akon
AU - Watanabe, Toru
AU - Noguchi, Yusuke
AU - Chang, Yung
AU - Chen, Wen Yih
AU - Matsuoka, Yuki
N1 - Funding Information:
Acknowledgments This research was partially supported from a Grants-in-Aid for Scientific Research (No. 14655136 and 17560691) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan. This work was also supported by ‘‘High-Tech Research Center’’ Project from the Ministry of Education, Culture, Sports, Science, and Technology of Japan. Asahi Glass Foundation, Iketani Science Technology Foundation, Salt Science Foundation, and National Science Council of Taiwan under the Grant No.
PY - 2007/7
Y1 - 2007/7
N2 - The neurite outgrowth of PC12 cells on collagen-coated glass plates under light emitting diode (LED) irradiation at several wavelengths (i.e., 455, 470, 525, 600, 630, 880 and 945 nm) was investigated. No neurite outgrowth was observed during cultivation under irradiation from the lamp of an inverted light microscope through filters (yielding mixed light at ca. 525 nm and more than 800 nm), whereas neurite outgrowth was observed during cultivation in the dark. When these cells were irradiated with monochromatic LED light, neurite outgrowth was slightly, but not completely, suppressed at 455, 525, 600, 630, 880 and 945 nm, as was observed in the case of mixed light. Long connected neuronal outgrowths (e.g., 3 mm length) were observed with LED light at 470 nm and 1.8 mW/cm2 intensity. No such outgrowths were observed at other LED light wavelengths (i.e., 455, 525, 600, 630, 880 and 945 nm). Irradiation at 470 nm may have caused specific responses to transductional signals in these cells that led to the connection of neuronal outgrowths between cells. Not only suppressed neurite outgrowth but also long connected neurite outgrowths were observed when PC12 cells were cultured under several different wavelengths of light.
AB - The neurite outgrowth of PC12 cells on collagen-coated glass plates under light emitting diode (LED) irradiation at several wavelengths (i.e., 455, 470, 525, 600, 630, 880 and 945 nm) was investigated. No neurite outgrowth was observed during cultivation under irradiation from the lamp of an inverted light microscope through filters (yielding mixed light at ca. 525 nm and more than 800 nm), whereas neurite outgrowth was observed during cultivation in the dark. When these cells were irradiated with monochromatic LED light, neurite outgrowth was slightly, but not completely, suppressed at 455, 525, 600, 630, 880 and 945 nm, as was observed in the case of mixed light. Long connected neuronal outgrowths (e.g., 3 mm length) were observed with LED light at 470 nm and 1.8 mW/cm2 intensity. No such outgrowths were observed at other LED light wavelengths (i.e., 455, 525, 600, 630, 880 and 945 nm). Irradiation at 470 nm may have caused specific responses to transductional signals in these cells that led to the connection of neuronal outgrowths between cells. Not only suppressed neurite outgrowth but also long connected neurite outgrowths were observed when PC12 cells were cultured under several different wavelengths of light.
KW - Cell
KW - LED
KW - Nerve
KW - Neurite outgrowth
KW - Visible light
UR - http://www.scopus.com/inward/record.url?scp=34548356949&partnerID=8YFLogxK
U2 - 10.1007/s10616-007-9087-y
DO - 10.1007/s10616-007-9087-y
M3 - 期刊論文
C2 - 19003010
AN - SCOPUS:34548356949
SN - 0920-9069
VL - 54
SP - 181
EP - 188
JO - Cytotechnology
JF - Cytotechnology
IS - 3
ER -