Using isothermal titration calorimetry to real-time monitor the heat of metabolism: A case study using PC12 cells and Aβ(1-40)

Steven S.S. Wang, Ming Shen Lin, Shen Liang Chen, Yung Chang, Rouch Chyu Ruaan, Wen Yih Chen

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Isothermal titration calorimetry (ITC) is one of the most powerful means for direct determination of thermodynamic information associated with most physiochemical and biological processes. The deposition and aggregation of β-amyloid (Aβ) on cell membranes was considered as one of the primary factors in having Alzheimer's disease (AD). Recently, a growing body of evidence has suggested that plasma membrane could accelerate the accumulation of Aβ on the plasma membranes. However, the mechanism of AD is still a controversial issue. This study provided a biothermodynamic approach to real-time monitor the heat of metabolism involved in the co-incubation of PC12 cells and Aβ(1-40) by ITC. The effects of Aβ conformation and concentration of oligomeric Aβ on cytotoxicity were successfully distinguished by ITC. This approach with rapid and direct measurement may provide not only real-time information for the effects of Aβ species on living cells but also a platform for the screening of drug candidates for AD.

Original languageEnglish
Pages (from-to)307-312
Number of pages6
JournalColloids and Surfaces B: Biointerfaces
Volume83
Issue number2
DOIs
StatePublished - 1 Apr 2011

Keywords

  • β-Amyloid
  • Alzheimer's disease
  • Biothermodynamics
  • Isothermal titration calorimetry

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