Use of liposomal amplifiers in total internal reflection fluorescence fiber-optic biosensors for protein detection

Ying Feng Chang, Chen Fu, Yi Ting Chen, Amily Fang-Ju Jou, Chii Chang Chen, Chien Chou, Ja An Annie Ho

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Evanescent-wave excited fluorescence technology has been demonstrated to enhance sensitivity and reduce matrix effects, making it suitable for biosensor development. In this study, we developed a liposome-based, total internal reflection fluorescence, fiber-optic biosensor (TIRF-FOB) for protein detection, which integrates a liposomal amplifier and sandwich immunoassay format with TIRF-FOB. In addition, the antibody-tagged and fluorophore-entrapped liposomes for heterogeneous detection of target molecules were designed and synthesized. This biosensor successfully detected the target protein (model analyzed here is IgG) with a limit of detection (LOD) of 2.0 attomoles for the target protein (equivalent to 2.0. pg/mL of protein presented in 150. μL of sample solution). The features of this ultra-sensitive liposomal TIRF-FOB are (i) fluorescence is excited via evanescent waves and amplified via liposomes; (ii) the use of two polyclonal antibodies in the sandwich assay format increases the specificity and lowers the cost of our assay. Based on the exceptional detection sensitivity and cost-effectiveness, we believe that the proposed biosensor has great potential as a practical, clinical diagnostic tool in the near future.

Original languageEnglish
Pages (from-to)1201-1207
Number of pages7
JournalBiosensors and Bioelectronics
Volume77
DOIs
StatePublished - 15 Mar 2016

Keywords

  • Evanescent wave-excited fluorescence
  • Fiber-optic biosensors
  • Liposomes
  • Protein detection

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