Use of biotinylated chitosan for substrate-mediated gene delivery

Wei Wen Hu, Wun Jheng Syu, Wen Yih Chen, Ruoh Chyu Ruaan, Yu Che Cheng, Chih Cheng Chien, Chuan Li, Chih Ang Chung, Chia Wen Tsao

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


To improve transfection efficiency of nonviral vectors, biotinylated chitosan was applied to complex with DNA in different N/P ratios. The morphologies and the sizes of formed nanoparticles were suitable for cell uptake. The biotinylation decreased the surface charges of nanoparticles and hence reduced the cytotoxicity. The loading capacities of chitosan were slightly decreased with the increase of biotinylation, but most of the DNA molecules were still complexed. Using different avidin-coated surfaces, the interaction between biotinylated nanoparticles to the substrate may be manipulated. The in vitro transfection results demonstrated that biotinylated nanoparticles may be bound to avidin coated surfaces, and the transfection efficiencies were thus increased. Through regulating the N/P ratio, biotinylation levels, and surface avidin, the gene delivery can be optimized. Compared to the nonmodified chitosan, biotinylated nanoparticles on biomaterial surfaces can increase their chances to contact adhered cells. This spatially controlled gene delivery improved the gene transfer efficiency of nonviral vectors and could be broadly applied to different biomaterial scaffolds for tissue engineering applications.

Original languageEnglish
Pages (from-to)1587-1599
Number of pages13
JournalBioconjugate Chemistry
Issue number8
StatePublished - 15 Aug 2012


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