TY - JOUR
T1 - Unveiling the topographic cue rendered by micropatterns for steering cell differentiation by using extrinsic photobiomodulation
AU - Tsai, Guan Ying
AU - Klom-In, Thipwadee
AU - Wang, Meng Jiy
AU - Chen, Szu yuan
N1 - Publisher Copyright:
© 2024
PY - 2024/4
Y1 - 2024/4
N2 - It is known that cell culture micropatterns have the ability to facilitate stem cell differentiation induced by specialized chemical factors and different differentiation directions have different optimal micropattern shapes. In this study, by utilizing extrinsic photobiomodulation (EPM) with verteporfin as photosensitizer and light irradiation of 690 nm wavelength as a universal, unbiased, and synchronizing way of inducing differentiation of human umbilical cord Wharton's Jelly mesenchymal stem cells (WJ-MSCs), the topographic cue for cell type specification conveyed in microislands is investigated. It is found that the topographic cues are encoded in the symmetry and aspect ratio of microislands and conformation of cells to microislands is necessary for acquiring the cue. F-actin vertical columns form and cell thickness increases for cells on microislands, and the two effects are enhanced by EPM and correlate with cell differentiation. EPM treatment poises cells in a stationary state to initiate differentiation and the process of making commitment takes two days. Our findings reveal the way to fully exploit topographic cues for promoting and controlling cell differentiation.
AB - It is known that cell culture micropatterns have the ability to facilitate stem cell differentiation induced by specialized chemical factors and different differentiation directions have different optimal micropattern shapes. In this study, by utilizing extrinsic photobiomodulation (EPM) with verteporfin as photosensitizer and light irradiation of 690 nm wavelength as a universal, unbiased, and synchronizing way of inducing differentiation of human umbilical cord Wharton's Jelly mesenchymal stem cells (WJ-MSCs), the topographic cue for cell type specification conveyed in microislands is investigated. It is found that the topographic cues are encoded in the symmetry and aspect ratio of microislands and conformation of cells to microislands is necessary for acquiring the cue. F-actin vertical columns form and cell thickness increases for cells on microislands, and the two effects are enhanced by EPM and correlate with cell differentiation. EPM treatment poises cells in a stationary state to initiate differentiation and the process of making commitment takes two days. Our findings reveal the way to fully exploit topographic cues for promoting and controlling cell differentiation.
KW - Adipogenic differentiation
KW - Collagen microisland
KW - Extrinsic photobiomodulation
KW - F-actin vertical column
KW - Mesenchymal stem cells
KW - Osteogenic differentiation
UR - http://www.scopus.com/inward/record.url?scp=85183489414&partnerID=8YFLogxK
U2 - 10.1016/j.jpap.2024.100229
DO - 10.1016/j.jpap.2024.100229
M3 - 期刊論文
AN - SCOPUS:85183489414
SN - 2666-4690
VL - 20
JO - Journal of Photochemistry and Photobiology
JF - Journal of Photochemistry and Photobiology
M1 - 100229
ER -