Universal behavior of membranes with sterols

J. Henriksen; A. C. Rowat; E. Brief; Y. W. Hsueh; J. L. Thewalt; M. J. Zuckermann; J. H. Ipsen

doi:10.1529/biophysj.105.067652

Universal behavior of membranes with sterols

J. Henriksen, A. C. Rowat, E. Brief, Y. W. Hsueh, J. L. Thewalt, M. J. Zuckermann, J. H. Ipsen

Department of Physics

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225 Scopus citations

Abstract

Lanosterol is the biosynthetic precursor of cholesterol and ergosterol, sterols that predominate in the membranes of mammals and lower eukaryotes, respectively. These three sterols are structurally quite similar, yet their relative effects on membranes have been shown to differ. Here we study the effects of cholesterol, lanosterol, and ergosterol on 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine lipid bilayers at room temperature. Micropipette aspiration is used to determine membrane material properties (area compressibility modulus), and information about lipid chain order (first moments) is obtained from deuterium nuclear magnetic resonance. We compare these results, along with data for membrane-bending rigidity, to explore the relationship between membrane hydrophobic thickness and elastic properties. Together, such diverse approaches demonstrate that membrane properties are affected to different degrees by these structurally distinct sterols, yet nonetheless exhibit universal behavior.

Original language	English
Pages (from-to)	1639-1649
Number of pages	11
Journal	Biophysical Journal
Volume	90
Issue number	5
DOIs	https://doi.org/10.1529/biophysj.105.067652
State	Published - Mar 2006

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title = "Universal behavior of membranes with sterols",

abstract = "Lanosterol is the biosynthetic precursor of cholesterol and ergosterol, sterols that predominate in the membranes of mammals and lower eukaryotes, respectively. These three sterols are structurally quite similar, yet their relative effects on membranes have been shown to differ. Here we study the effects of cholesterol, lanosterol, and ergosterol on 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine lipid bilayers at room temperature. Micropipette aspiration is used to determine membrane material properties (area compressibility modulus), and information about lipid chain order (first moments) is obtained from deuterium nuclear magnetic resonance. We compare these results, along with data for membrane-bending rigidity, to explore the relationship between membrane hydrophobic thickness and elastic properties. Together, such diverse approaches demonstrate that membrane properties are affected to different degrees by these structurally distinct sterols, yet nonetheless exhibit universal behavior.",

author = "J. Henriksen and Rowat, {A. C.} and E. Brief and Hsueh, {Y. W.} and Thewalt, {J. L.} and Zuckermann, {M. J.} and Ipsen, {J. H.}",

note = "Funding Information: MEMPHYS - Centre for Membrane Biophysics is supported by the Danish National Research Foundation. A.C.R. is supported by a Julie Payette Scholarship from the National Research Council of Canada. Y.H. received support from National Science Council of Taiwan (grant No. NSC 92-2112-M-008-049). ",

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AU - Henriksen, J.

AU - Rowat, A. C.

AU - Brief, E.

AU - Hsueh, Y. W.

AU - Thewalt, J. L.

AU - Zuckermann, M. J.

AU - Ipsen, J. H.

N1 - Funding Information: MEMPHYS - Centre for Membrane Biophysics is supported by the Danish National Research Foundation. A.C.R. is supported by a Julie Payette Scholarship from the National Research Council of Canada. Y.H. received support from National Science Council of Taiwan (grant No. NSC 92-2112-M-008-049).

PY - 2006/3

Y1 - 2006/3

N2 - Lanosterol is the biosynthetic precursor of cholesterol and ergosterol, sterols that predominate in the membranes of mammals and lower eukaryotes, respectively. These three sterols are structurally quite similar, yet their relative effects on membranes have been shown to differ. Here we study the effects of cholesterol, lanosterol, and ergosterol on 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine lipid bilayers at room temperature. Micropipette aspiration is used to determine membrane material properties (area compressibility modulus), and information about lipid chain order (first moments) is obtained from deuterium nuclear magnetic resonance. We compare these results, along with data for membrane-bending rigidity, to explore the relationship between membrane hydrophobic thickness and elastic properties. Together, such diverse approaches demonstrate that membrane properties are affected to different degrees by these structurally distinct sterols, yet nonetheless exhibit universal behavior.

AB - Lanosterol is the biosynthetic precursor of cholesterol and ergosterol, sterols that predominate in the membranes of mammals and lower eukaryotes, respectively. These three sterols are structurally quite similar, yet their relative effects on membranes have been shown to differ. Here we study the effects of cholesterol, lanosterol, and ergosterol on 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine lipid bilayers at room temperature. Micropipette aspiration is used to determine membrane material properties (area compressibility modulus), and information about lipid chain order (first moments) is obtained from deuterium nuclear magnetic resonance. We compare these results, along with data for membrane-bending rigidity, to explore the relationship between membrane hydrophobic thickness and elastic properties. Together, such diverse approaches demonstrate that membrane properties are affected to different degrees by these structurally distinct sterols, yet nonetheless exhibit universal behavior.

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M3 - 期刊論文

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SN - 0006-3495

VL - 90

SP - 1639

EP - 1649

JO - Biophysical Journal

JF - Biophysical Journal

IS - 5

ER -

Universal behavior of membranes with sterols

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