Ultrasensitive Detection of Interleukin 6 by Using Silicon Nanowire Field-Effect Transistors

Wen Pin Hu, Yu Ming Wu, Cao An Vu, Wen Yih Chen

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Interleukin 6 (IL-6) has been regarded as a biomarker that can be applied as a predictor for the severity of COVID-19-infected patients. The IL-6 level also correlates well with respiratory dysfunction and mortality risk. In this work, three silanization approaches and two types of biorecognition elements were used on the silicon nanowire field-effect transistors (SiNW-FETs) to investigate and compare the sensing performance on the detection of IL-6. Experimental data revealed that the mixed-SAMs-modified silica surface could have superior surface morphology to APTES-modified and APS-modified silica surfaces. According to the data on detecting various concentrations of IL-6, the detection range of the aptamer-functionalized SiNW-FET was broader than that of the antibody-functionalized SiNW-FET. In addition, the lowest concentration of valid detection for the aptamer-functionalized SiNW-FET was 2.1 pg/mL, two orders of magnitude lower than the antibody-functionalized SiNW-FET. The detection range of the aptamer-functionalized SiNW-FET covered the concentration of IL-6, which could be used to predict fatal outcomes of COVID-19. The detection results in the buffer showed that the anti-IL-6 aptamer could produce better detection results on the SiNW-FETs, indicating its great opportunity in applications for sensing clinical samples.

Original languageEnglish
Article number625
JournalSensors (Switzerland)
Volume23
Issue number2
DOIs
StatePublished - Jan 2023

Keywords

  • aptamer
  • COVID-19
  • field-effect transistors
  • infectious diseases
  • interleukin 6

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