Tubular optical waveguide particle plasmon resonance biosensor for multiplex real-time and label-free detection

Chen Han Huang, Hsing Ying Lin, Lai Kwan Chau

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


A tubular optical waveguide particle plasmon resonance (TW-PPR) sensor is demonstrated for higher-throughput and sensitive label-free biochemical detections. Compared to other evanescent field absorption sensors, the TW-PPR sensor possesses merits of itself being a microchamber of a defined sample volume, a mechanical support for sensor coatings, and ease of systematic multichannel expansion. The sensor resolution is estimated to be 2.6 × 10-6 RIU in measuring solutions of various refractive indices (RIs). Additionally, the multichannel TW-PPR sensing system can perform independent measurements simultaneously and its limit of detection (LOD) of anti-DNP antibody and streptavidin separately measured by DNP-functionalized and biotin-functionalized TW-PPR microchambers is demonstrated to be 1.21 × 10-10 and 2.27 × 10-10 g/ml, respectively. Accurate determinations of these molecules with known concentrations spiked in artificial urine are examined and the sensor responses give excellent correlation with results demonstrated in standard buffer examinations, supporting the utility of the device for analyte screening in more complex media. The TWPPR sensor can be inexpensively fabricated and has a special niche as high-sensitivity refractive index sensor as well as biosensor for label-free monitoring biomolecular interactions in real-time. It is ideally suitable for disposable uses, especially promising for convenient higher-throughput biochemical sensing applications.

Original languageEnglish
Title of host publicationOptical Sensors 2013
StatePublished - 2013
EventOptical Sensors 2013 - Prague, Czech Republic
Duration: 15 Apr 201317 Apr 2013

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X


ConferenceOptical Sensors 2013
Country/TerritoryCzech Republic


  • Biosensor
  • High-throughput biochemical detection
  • Localized surface plasmon resonance
  • Nanoparticle
  • Optical waveguide


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