TY - JOUR
T1 - Towards Bio-Encapsulation of Chitosan-Silver Nanocomplex? Impact on Malaria Mosquito Vectors, Human Breast Adenocarcinoma Cells (MCF-7) and Behavioral Traits of Non-target Fishes
AU - Murugan, Kadarkarai
AU - Jaganathan, Anitha
AU - Suresh, Udaiyan
AU - Rajaganesh, Rajapandian
AU - Jayasanthini, Sudalaimani
AU - Higuchi, Akon
AU - Kumar, Suresh
AU - Benelli, Giovanni
N1 - Publisher Copyright:
© 2016, Springer Science+Business Media New York.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - In this study, we synthesized and bio-encapsulated a chitosan-silver nanocomplex (Ch-AgNPs), characterizing it by UV–Vis spectroscopy, FTIR, EDX, SEM, XRD and Zeta potential analyses. The bio-encapsulated chitosan-Ag nanocomplex (BNC) was efficient as scavenger of free radicals (DPPH and ABTS), if compared to Ch-AgNPs. In toxicity assays against breast cancer cells (MCF-7) the BNC triggered apoptotic pathways, leading to a decline of MCF-7 cell viability with IC50of 17.79 μg/mL after 48 h of exposure. LC50of BNC on Anopheles stephensi ranged from 54.65 (larva I), to 98.172 ppm (pupa) while Ch-AgNPs LC50ranged from 4.432 (I) to 7.641 ppm (pupa). In the field, the application of Ch-AgNP (10 × LC50) lead to A. stephensi larval reduction to 86.2, 48.4 and 100% after 24, 48, and 72 h, while the BNC nanocomplex exhibited 68.8, 36.4 and 100% larval reduction, respectively. Both Ch-AgNPs and the BNC reduced longevity and fecundity of A. stephensi. As regards to non-target effects on fish behavioral traits, in standard conditions, Poecilia reticulata predation on A. stephensi larvae was 70.25 (II) and 46.75 larvae per day (III), while post-treatment with sub-lethal doses of BNC, predation was boosted to 88.5 (II) and 70.25 (III) larvae per day.
AB - In this study, we synthesized and bio-encapsulated a chitosan-silver nanocomplex (Ch-AgNPs), characterizing it by UV–Vis spectroscopy, FTIR, EDX, SEM, XRD and Zeta potential analyses. The bio-encapsulated chitosan-Ag nanocomplex (BNC) was efficient as scavenger of free radicals (DPPH and ABTS), if compared to Ch-AgNPs. In toxicity assays against breast cancer cells (MCF-7) the BNC triggered apoptotic pathways, leading to a decline of MCF-7 cell viability with IC50of 17.79 μg/mL after 48 h of exposure. LC50of BNC on Anopheles stephensi ranged from 54.65 (larva I), to 98.172 ppm (pupa) while Ch-AgNPs LC50ranged from 4.432 (I) to 7.641 ppm (pupa). In the field, the application of Ch-AgNP (10 × LC50) lead to A. stephensi larval reduction to 86.2, 48.4 and 100% after 24, 48, and 72 h, while the BNC nanocomplex exhibited 68.8, 36.4 and 100% larval reduction, respectively. Both Ch-AgNPs and the BNC reduced longevity and fecundity of A. stephensi. As regards to non-target effects on fish behavioral traits, in standard conditions, Poecilia reticulata predation on A. stephensi larvae was 70.25 (II) and 46.75 larvae per day (III), while post-treatment with sub-lethal doses of BNC, predation was boosted to 88.5 (II) and 70.25 (III) larvae per day.
KW - Biological control
KW - Biosafety
KW - MCF-7
KW - Nanoparticle stability
UR - http://www.scopus.com/inward/record.url?scp=84997719943&partnerID=8YFLogxK
U2 - 10.1007/s10876-016-1129-1
DO - 10.1007/s10876-016-1129-1
M3 - 期刊論文
AN - SCOPUS:84997719943
SN - 1040-7278
VL - 28
SP - 529
EP - 550
JO - Journal of Cluster Science
JF - Journal of Cluster Science
IS - 1
ER -