Toll-like receptor 6 and connective tissue growth factor are significantly upregulated in mitomycin-C-treated urothelial carcinoma cells under hydrostatic pressure stimulation

Shao Kuan Chen, Chih Ang Chung, Yu Che Cheng, Chi Jung Huang, Wen Yih Chen, Ruoh Chyu Ruaan, Chuan Li, Chia Wen Tsao, Wei Wen Hu, Chih Cheng Chien

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Background: Urothelial carcinoma (UC) is the most common histologic subtype of bladder cancer. The administration of mitomycin C (MMC) into the bladder after transurethral resection of the bladder tumor (TURBT) is a common treatment strategy for preventing recurrence after surgery. We previously applied hydrostatic pressure combined with MMC in UC cells and found that hydrostatic pressure synergistically enhanced MMC-induced UC cell apoptosis through the Fas/FasL pathways. To understand the alteration of gene expressions in UC cells caused by hydrostatic pressure and MMC, oligonucleotide microarray was used to explore all the differentially expressed genes. Results: After bioinformatics analysis and gene annotation, Toll-like receptor 6 (TLR6) and connective tissue growth factor (CTGF) showed significant upregulation among altered genes, and their gene and protein expressions with each treatment of UC cells were validated by quantitative real-time PCR and immunoblotting. Conclusion: Under treatment with MMC and hydrostatic pressure, UC cells showed increasing apoptosis using extrinsic pathways through upregulation of TLR6 and CTGF.

Original languageEnglish
Pages (from-to)410-416
Number of pages7
JournalGenetic Testing and Molecular Biomarkers
Volume18
Issue number6
DOIs
StatePublished - 1 Jun 2014

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