Toll-like receptor 3 expression inhibits cell invasion and migration and predicts a favorable prognosis in neuroblastoma

Wen Ming Hsu; Chao Cheng Huang; Pei Yi Wu; Hsinyu Lee; Min Chuan Huang; Ming Hong Tai; Jiin Haur Chuang

doi:10.1016/j.canlet.2013.03.024

Toll-like receptor 3 expression inhibits cell invasion and migration and predicts a favorable prognosis in neuroblastoma

Wen Ming Hsu, Chao Cheng Huang, Pei Yi Wu, Hsinyu Lee, Min Chuan Huang, Ming Hong Tai, Jiin Haur Chuang

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

To evaluate the clinical significance of TLR3 expression on neuroblastomas, we performed immunohistochemical study on archival tissues and in vitro studies on neuroblastoma cell lines. The results showed that positive TLR3 expression was associated with favorable histology and prognosis. Activation of TLR3 by polyinosinic:polycytidylic acid [poly(I:C)] treatment is effective to suppress cell migration and invasion and to decrease organized assembly of F-actin and filopodia formation, in TLR3-expressing SK-N-AS cells, which could be reversed by TLR3-targeting siRNA treatment. TLR3 agonist poly(I:C) promotes GAP-43 expression also in SK-N-AS cells only. Taken together, TLR3 could serve to predict favorable behavior in neuroblastomas.

Original language	English
Pages (from-to)	338-346
Number of pages	9
Journal	Cancer Letters
Volume	336
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2013.03.024
State	Published - 19 Aug 2013

Keywords

Migration
Neuroblastoma
Poly(I:C)
Prognosis
Toll-like receptor 3

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.canlet.2013.03.024

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title = "Toll-like receptor 3 expression inhibits cell invasion and migration and predicts a favorable prognosis in neuroblastoma",

abstract = "To evaluate the clinical significance of TLR3 expression on neuroblastomas, we performed immunohistochemical study on archival tissues and in vitro studies on neuroblastoma cell lines. The results showed that positive TLR3 expression was associated with favorable histology and prognosis. Activation of TLR3 by polyinosinic:polycytidylic acid [poly(I:C)] treatment is effective to suppress cell migration and invasion and to decrease organized assembly of F-actin and filopodia formation, in TLR3-expressing SK-N-AS cells, which could be reversed by TLR3-targeting siRNA treatment. TLR3 agonist poly(I:C) promotes GAP-43 expression also in SK-N-AS cells only. Taken together, TLR3 could serve to predict favorable behavior in neuroblastomas.",

keywords = "Migration, Neuroblastoma, Poly(I:C), Prognosis, Toll-like receptor 3",

author = "Hsu, {Wen Ming} and Huang, {Chao Cheng} and Wu, {Pei Yi} and Hsinyu Lee and Huang, {Min Chuan} and Tai, {Ming Hong} and Chuang, {Jiin Haur}",

note = "Funding Information: The authors wish to thank Ms. Chia-Ling Wu and Chang Gung Medical Foundation Kaohsiung Chang Gung Memorial Hospital Tissue Bank (CLRPG8B0031) for excellent technical support, and Vanessa Yu of Taiwan Instrument Co., Ltd., for her assistance in using confocal laser microscopy. This study was supported by the Grants from the Chang Gung Memorial Hospital (CMRPG890303 & CMRPG891091). ",

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T1 - Toll-like receptor 3 expression inhibits cell invasion and migration and predicts a favorable prognosis in neuroblastoma

AU - Hsu, Wen Ming

AU - Huang, Chao Cheng

AU - Wu, Pei Yi

AU - Lee, Hsinyu

AU - Huang, Min Chuan

AU - Tai, Ming Hong

AU - Chuang, Jiin Haur

N1 - Funding Information: The authors wish to thank Ms. Chia-Ling Wu and Chang Gung Medical Foundation Kaohsiung Chang Gung Memorial Hospital Tissue Bank (CLRPG8B0031) for excellent technical support, and Vanessa Yu of Taiwan Instrument Co., Ltd., for her assistance in using confocal laser microscopy. This study was supported by the Grants from the Chang Gung Memorial Hospital (CMRPG890303 & CMRPG891091).

PY - 2013/8/19

Y1 - 2013/8/19

N2 - To evaluate the clinical significance of TLR3 expression on neuroblastomas, we performed immunohistochemical study on archival tissues and in vitro studies on neuroblastoma cell lines. The results showed that positive TLR3 expression was associated with favorable histology and prognosis. Activation of TLR3 by polyinosinic:polycytidylic acid [poly(I:C)] treatment is effective to suppress cell migration and invasion and to decrease organized assembly of F-actin and filopodia formation, in TLR3-expressing SK-N-AS cells, which could be reversed by TLR3-targeting siRNA treatment. TLR3 agonist poly(I:C) promotes GAP-43 expression also in SK-N-AS cells only. Taken together, TLR3 could serve to predict favorable behavior in neuroblastomas.

AB - To evaluate the clinical significance of TLR3 expression on neuroblastomas, we performed immunohistochemical study on archival tissues and in vitro studies on neuroblastoma cell lines. The results showed that positive TLR3 expression was associated with favorable histology and prognosis. Activation of TLR3 by polyinosinic:polycytidylic acid [poly(I:C)] treatment is effective to suppress cell migration and invasion and to decrease organized assembly of F-actin and filopodia formation, in TLR3-expressing SK-N-AS cells, which could be reversed by TLR3-targeting siRNA treatment. TLR3 agonist poly(I:C) promotes GAP-43 expression also in SK-N-AS cells only. Taken together, TLR3 could serve to predict favorable behavior in neuroblastomas.

KW - Migration

KW - Neuroblastoma

KW - Poly(I:C)

KW - Prognosis

KW - Toll-like receptor 3

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U2 - 10.1016/j.canlet.2013.03.024

DO - 10.1016/j.canlet.2013.03.024

M3 - 期刊論文

C2 - 23541683

AN - SCOPUS:84879783063

SN - 0304-3835

VL - 336

SP - 338

EP - 346

JO - Cancer Letters

JF - Cancer Letters

IS - 2

ER -

Toll-like receptor 3 expression inhibits cell invasion and migration and predicts a favorable prognosis in neuroblastoma

Abstract

Keywords

UN SDGs

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