Thermoresponsive surfaces designed for the proliferation and differentiation of human pluripotent stem cells

Akon Higuchi, Abdurahman H. Hirad, S. Suresh Kumar, Murugan A. Munusamy, Abdullah A. Alarfaj

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Thermoresponsive surfaces enable the detachment of cells or cell sheets by decreasing the temperature of the surface when harvesting the cells. However, human pluripotent stem cells (hPSCs), such as embryonic stem cells and induced pluripotent stem cells, cannot be directly cultured on a thermoresponsive surface; hPSCs need a specific extracellular matrix to bind to the integrin receptors on their surfaces. We prepared a thermoresponsive surface by using poly(N-isopropylacrylamide-co-butylacrylate) and recombinant vitronectin to provide an optimal coating concentration for the hPSC culture. hPSCs can be cultured on the same thermoresponsive surface for 5 passages by partial detachment of the cells from the surface by decreasing the temperature for 30 min; then, the remaining hPSCs were subsequently cultured on the same dishes following the addition of new cultivation media. The detached cells, even after continual culture for five passages, showed high pluripotency, the ability to differentiate into cells derived from the 3 germ layers and the ability to undergo cardiac differentiation.

Original languageEnglish
Pages (from-to)162-173
Number of pages12
JournalActa Biomaterialia
Volume116
DOIs
StatePublished - 15 Oct 2020

Keywords

  • Biomaterial
  • Cardiomyocyte
  • Human pluripotent stem cell
  • Thermoresponsive polymer
  • Xeno-free culture

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