TY - JOUR
T1 - Thermoresponsive surfaces designed for the proliferation and differentiation of human pluripotent stem cells
AU - Higuchi, Akon
AU - Hirad, Abdurahman H.
AU - Kumar, S. Suresh
AU - Munusamy, Murugan A.
AU - Alarfaj, Abdullah A.
N1 - Publisher Copyright:
© 2020
PY - 2020/10/15
Y1 - 2020/10/15
N2 - Thermoresponsive surfaces enable the detachment of cells or cell sheets by decreasing the temperature of the surface when harvesting the cells. However, human pluripotent stem cells (hPSCs), such as embryonic stem cells and induced pluripotent stem cells, cannot be directly cultured on a thermoresponsive surface; hPSCs need a specific extracellular matrix to bind to the integrin receptors on their surfaces. We prepared a thermoresponsive surface by using poly(N-isopropylacrylamide-co-butylacrylate) and recombinant vitronectin to provide an optimal coating concentration for the hPSC culture. hPSCs can be cultured on the same thermoresponsive surface for 5 passages by partial detachment of the cells from the surface by decreasing the temperature for 30 min; then, the remaining hPSCs were subsequently cultured on the same dishes following the addition of new cultivation media. The detached cells, even after continual culture for five passages, showed high pluripotency, the ability to differentiate into cells derived from the 3 germ layers and the ability to undergo cardiac differentiation.
AB - Thermoresponsive surfaces enable the detachment of cells or cell sheets by decreasing the temperature of the surface when harvesting the cells. However, human pluripotent stem cells (hPSCs), such as embryonic stem cells and induced pluripotent stem cells, cannot be directly cultured on a thermoresponsive surface; hPSCs need a specific extracellular matrix to bind to the integrin receptors on their surfaces. We prepared a thermoresponsive surface by using poly(N-isopropylacrylamide-co-butylacrylate) and recombinant vitronectin to provide an optimal coating concentration for the hPSC culture. hPSCs can be cultured on the same thermoresponsive surface for 5 passages by partial detachment of the cells from the surface by decreasing the temperature for 30 min; then, the remaining hPSCs were subsequently cultured on the same dishes following the addition of new cultivation media. The detached cells, even after continual culture for five passages, showed high pluripotency, the ability to differentiate into cells derived from the 3 germ layers and the ability to undergo cardiac differentiation.
KW - Biomaterial
KW - Cardiomyocyte
KW - Human pluripotent stem cell
KW - Thermoresponsive polymer
KW - Xeno-free culture
UR - http://www.scopus.com/inward/record.url?scp=85091257259&partnerID=8YFLogxK
U2 - 10.1016/j.actbio.2020.09.010
DO - 10.1016/j.actbio.2020.09.010
M3 - 期刊論文
C2 - 32911107
AN - SCOPUS:85091257259
SN - 1742-7061
VL - 116
SP - 162
EP - 173
JO - Acta Biomaterialia
JF - Acta Biomaterialia
ER -