Thermodynamics and mechanism of ssDNA hybridization below the melting temperature by isothermal titration calorimetry

An Cheng Liu; Liang Yu Chen; Chung Fan Chiou; Hung Ju Richard Su; Yu Chia Chan; Pei Hsuan Tasi; Shean Jen Chen; Wen Yih Chen

doi:10.1016/j.tca.2005.01.072

Thermodynamics and mechanism of ssDNA hybridization below the melting temperature by isothermal titration calorimetry

An Cheng Liu, Liang Yu Chen, Chung Fan Chiou, Hung Ju Richard Su, Yu Chia Chan, Pei Hsuan Tasi, Shean Jen Chen, Wen Yih Chen

Department of Chemical and Materials Engineering

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

This investigation presents measurements of isothermal ssDNA oligomer hybridization enthalpy at various DNA length, GC contents, temperatures, and salt concentrations by isothermal titration calorimetry (ITC) at temperatures below the melting temperature of the DNA. The study aimed to reveal the hybridization mechanism of ssDNA for gene chip applications. The role of hydrogen bonds between complementary bases, the stacking forces between bases, the electrostatic repulsion between strands, the dehydration and conformational rearrangement of ssDNA in hybridization are estimated from the interaction enthalpy.

Original language	English
Pages (from-to)	83-87
Number of pages	5
Journal	Thermochimica Acta
Volume	433
Issue number	1-2
DOIs	https://doi.org/10.1016/j.tca.2005.01.072
State	Published - 1 Aug 2005

Keywords

DNA hybridization
Enthalpy
Gene chip
Isothermal titration calorimetry

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10.1016/j.tca.2005.01.072

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title = "Thermodynamics and mechanism of ssDNA hybridization below the melting temperature by isothermal titration calorimetry",

abstract = "This investigation presents measurements of isothermal ssDNA oligomer hybridization enthalpy at various DNA length, GC contents, temperatures, and salt concentrations by isothermal titration calorimetry (ITC) at temperatures below the melting temperature of the DNA. The study aimed to reveal the hybridization mechanism of ssDNA for gene chip applications. The role of hydrogen bonds between complementary bases, the stacking forces between bases, the electrostatic repulsion between strands, the dehydration and conformational rearrangement of ssDNA in hybridization are estimated from the interaction enthalpy.",

keywords = "DNA hybridization, Enthalpy, Gene chip, Isothermal titration calorimetry",

author = "Liu, {An Cheng} and Chen, {Liang Yu} and Chiou, {Chung Fan} and Su, {Hung Ju Richard} and Chan, {Yu Chia} and Tasi, {Pei Hsuan} and Chen, {Shean Jen} and Chen, {Wen Yih}",

note = "Funding Information: The authors would like to thank the National Science Council of the Republic of China, Taiwan under Contract No. NSC 92-2214-E-008-003, and the Biomedical Engineering Center, Industrial Technology Research Institute of Taiwan for financially supporting this research.",

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T1 - Thermodynamics and mechanism of ssDNA hybridization below the melting temperature by isothermal titration calorimetry

AU - Liu, An Cheng

AU - Chen, Liang Yu

AU - Chiou, Chung Fan

AU - Su, Hung Ju Richard

AU - Chan, Yu Chia

AU - Tasi, Pei Hsuan

AU - Chen, Shean Jen

AU - Chen, Wen Yih

N1 - Funding Information: The authors would like to thank the National Science Council of the Republic of China, Taiwan under Contract No. NSC 92-2214-E-008-003, and the Biomedical Engineering Center, Industrial Technology Research Institute of Taiwan for financially supporting this research.

PY - 2005/8/1

Y1 - 2005/8/1

N2 - This investigation presents measurements of isothermal ssDNA oligomer hybridization enthalpy at various DNA length, GC contents, temperatures, and salt concentrations by isothermal titration calorimetry (ITC) at temperatures below the melting temperature of the DNA. The study aimed to reveal the hybridization mechanism of ssDNA for gene chip applications. The role of hydrogen bonds between complementary bases, the stacking forces between bases, the electrostatic repulsion between strands, the dehydration and conformational rearrangement of ssDNA in hybridization are estimated from the interaction enthalpy.

AB - This investigation presents measurements of isothermal ssDNA oligomer hybridization enthalpy at various DNA length, GC contents, temperatures, and salt concentrations by isothermal titration calorimetry (ITC) at temperatures below the melting temperature of the DNA. The study aimed to reveal the hybridization mechanism of ssDNA for gene chip applications. The role of hydrogen bonds between complementary bases, the stacking forces between bases, the electrostatic repulsion between strands, the dehydration and conformational rearrangement of ssDNA in hybridization are estimated from the interaction enthalpy.

KW - DNA hybridization

KW - Enthalpy

KW - Gene chip

KW - Isothermal titration calorimetry

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U2 - 10.1016/j.tca.2005.01.072

DO - 10.1016/j.tca.2005.01.072

M3 - 期刊論文

AN - SCOPUS:21244453058

SN - 0040-6031

VL - 433

SP - 83

EP - 87

JO - Thermochimica Acta

JF - Thermochimica Acta

IS - 1-2

ER -

Thermodynamics and mechanism of ssDNA hybridization below the melting temperature by isothermal titration calorimetry

Abstract

Keywords

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