The investigation of recognition interaction between phenylboronate monolayer and glycated hemoglobin using surface plasmon resonance

Jen Tsai Liu, Liang Yu Chen, Mu Chin Shih, Yung Chang, Wen Yih Chen

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

Glycated hemoglobin (HbA1c) is formed by a nonenzymatic reaction of glucose with the N-terminal valine of adult hemoglobin's β-chain. The amount of HbA1c reflects the average concentration of glucose variation level over the preceding 2 to 3 months. Because the boronate has antibody mimicking for HbA1c, often it is used to detect HbA1c. However, factors such as the ratio of the phenylboronic acid derivatives and diol composition, the pH of the solution, and the stereostructure of phenylboronic acid derivatives could influence the interactions between phenylboronic acid derivatives and diol composition. In this study, the factors were evaluated using surface plasmon resonance (SPR). The results show that pH value is an important factor affecting HbA1c and phenylboronic acid to form the complex and Lewis bases. This could change the stereostructure of phenylboronic acid to form B(OH)3 for binding with saccharine easily. In addition, linear response appeared in HbA1c in the range of 0.43 to 3.49 μg/ml, and the detection limit was 0.01 μg/ml. The results also demonstrated that an SPR biosensor can be used as a sensitive technique for improving the accuracy and correctness of HbA1c measurement.

Original languageEnglish
Pages (from-to)90-96
Number of pages7
JournalAnalytical Biochemistry
Volume375
Issue number1
DOIs
StatePublished - 1 Apr 2008

Keywords

  • Glycated hemoglobin (HbA1c)
  • Phenylboronic acid
  • Surface plasmon resonance (SPR)

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