The influence of NBD fluorescent probe on model membranes containing POPC and DPPC

Chi Jung Weng, Ju Ping Wu, Ming Yen Kuo, Ya Wei Hsueh

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4 Scopus citations

Abstract

To investigate the effect of fluorescent probe on the properties of membranes, we studied model membranes composed of 1,2- dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1-palmitoyl 2-oleoyl-sn-glycero-3-phosphocholine (POPC) in the presence and absence of fluorescent probe. The morphology of giant unilamellar vesicles (GUVs) has been observed as a function of temperature and composition by fluorescence microscopy using NBD-DOPE or C6-NBD-PC as the probe. The phase behavior of model membranes containing no fluorescent probe was investigated by 2H-NMR spectroscopy. We found that the bright phase observed on GUVs was the fluid phase enriched in POPC and the dark phase was the gel phase enriched in DPPC. NBD-DOPE and C6-NBD-PC preferentially participated in the fluid-phase domains when GUVs were in the gel + fluid phase coexistence. Inclusion of both fluorescent probes (1 mol%) lowered the transition temperature of POPC/DPPC membranes. In addition, C6-NBD-PC exhibited a stronger effect than NBD-DOPE, which was considered to be associated with the structures of fluorescent molecules.

Original languageEnglish
Pages (from-to)23-28
Number of pages6
JournalMolecular Membrane Biology
Volume33
Issue number1-2
DOIs
StatePublished - 17 Feb 2016

Keywords

  • 2H NMR
  • C6-NBD-PC
  • DPPC
  • fluorescence microscopy
  • NBD-DOPE
  • POPC

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