The effects of substrate-mediated electrical stimulation on the promotion of osteogenic differentiation and its optimization

Wei Wen Hu, Tun Chi Chen, Chia Wen Tsao, Yu Che Cheng

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


To explore the effect of electrical stimulation (ES) on osteogenesis, a polypyrrole (PPy)-made electrical culture system was developed to provide a direct-current electric field (DCEF). This DCEF device was applied to treat differentiated rat bone marrow stromal cells (rBMSCs) once in different stages of osteo-differentation to investigate its temporal effects. The mineralization results showed that the DCEF treatment not only accelerated cell differentiation but also promoted the saturation levels, and the ES on day 8 was the group demonstrated the optimal result. The gene regulation analysis indicated that the DCEF treatment immediately increased the levels of genes related to osteo-differentiation, especially Runx2. Because Runx2 is a crucial transcriptional factor of osteogenesis, the ES-caused improvement of mineralization was likely contributed by the extension of its expression. Further, different ES modes were investigated of their efficacy on bone matrix deposition. Square waves with different parameters including frequency, offset, amplitude, and duty cycle were systematically examined. In contrast to constant voltage, square waves demonstrated periodical changes of current through substrate to significantly improve mineralization, and the efficiencies highly depended on both frequency and intensity. Through this comprehensive study, DCEF treating condition was optimized, which should be beneficial to its application on osteogenesis promotion.

Original languageEnglish
Pages (from-to)1607-1619
Number of pages13
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Issue number5
StatePublished - Jul 2019


  • bone marrow stromal cells
  • direct-current electric field
  • osteogenesis
  • polypyrrole
  • substrate-mediated electrical stimulation


Dive into the research topics of 'The effects of substrate-mediated electrical stimulation on the promotion of osteogenic differentiation and its optimization'. Together they form a unique fingerprint.

Cite this