The effect of ceramide on phosphatidylcholine membranes: A deuterium NMR study

Ya Wei Hsueh; Ralph Giles; Neil Kitson; Jenifer Thewalt

doi:10.1016/S0006-3495(02)75650-6

The effect of ceramide on phosphatidylcholine membranes: A deuterium NMR study

Ya Wei Hsueh, Ralph Giles, Neil Kitson, Jenifer Thewalt

Department of Physics

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

Biological membranes contain domains having distinct physical properties. We study defined mixtures of phosphoglycerolipids and sphingolipids to ascertain the fundamental interactions governing these lipids in the absence of other cell membrane components. By using ²H-NMR we have determined the temperature and composition dependencies of membrane structure and phase behavior for aqueous dispersions of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and the ceramide (Cer) N-palmitoyl-sphingosine. It is found that gel and liquid-crystalline phases coexist over a wide range of temperature and composition. Domains of different composition and phase state are present in POPC/Cer membranes at physiological temperature for Cer concentrations exceeding 15 mol %. The acyl chains of liquid crystalline phase POPC are ordered by the presence of Cer. Moreover, Cer's chain ordering is greater than that of POPC in the liquid crystalline phase. However, there is no evidence of liquid-liquid phase separation in the liquid crystalline region of the POPC/Cer phase diagram.

Original language	English
Pages (from-to)	3089-3095
Number of pages	7
Journal	Biophysical Journal
Volume	82
Issue number	6
DOIs	https://doi.org/10.1016/S0006-3495(02)75650-6
State	Published - 2002

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title = "The effect of ceramide on phosphatidylcholine membranes: A deuterium NMR study",

abstract = "Biological membranes contain domains having distinct physical properties. We study defined mixtures of phosphoglycerolipids and sphingolipids to ascertain the fundamental interactions governing these lipids in the absence of other cell membrane components. By using 2H-NMR we have determined the temperature and composition dependencies of membrane structure and phase behavior for aqueous dispersions of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and the ceramide (Cer) N-palmitoyl-sphingosine. It is found that gel and liquid-crystalline phases coexist over a wide range of temperature and composition. Domains of different composition and phase state are present in POPC/Cer membranes at physiological temperature for Cer concentrations exceeding 15 mol %. The acyl chains of liquid crystalline phase POPC are ordered by the presence of Cer. Moreover, Cer's chain ordering is greater than that of POPC in the liquid crystalline phase. However, there is no evidence of liquid-liquid phase separation in the liquid crystalline region of the POPC/Cer phase diagram.",

author = "Hsueh, {Ya Wei} and Ralph Giles and Neil Kitson and Jenifer Thewalt",

note = "Funding Information: This work was supported by research grants from the Canadian Dermatology Foundation, the UBC Epidermolysis Bullosa Research Fund, and the National Sciences and Engineering Research Council of Canada.",

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T1 - The effect of ceramide on phosphatidylcholine membranes

T2 - A deuterium NMR study

AU - Hsueh, Ya Wei

AU - Giles, Ralph

AU - Kitson, Neil

AU - Thewalt, Jenifer

N1 - Funding Information: This work was supported by research grants from the Canadian Dermatology Foundation, the UBC Epidermolysis Bullosa Research Fund, and the National Sciences and Engineering Research Council of Canada.

PY - 2002

Y1 - 2002

N2 - Biological membranes contain domains having distinct physical properties. We study defined mixtures of phosphoglycerolipids and sphingolipids to ascertain the fundamental interactions governing these lipids in the absence of other cell membrane components. By using 2H-NMR we have determined the temperature and composition dependencies of membrane structure and phase behavior for aqueous dispersions of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and the ceramide (Cer) N-palmitoyl-sphingosine. It is found that gel and liquid-crystalline phases coexist over a wide range of temperature and composition. Domains of different composition and phase state are present in POPC/Cer membranes at physiological temperature for Cer concentrations exceeding 15 mol %. The acyl chains of liquid crystalline phase POPC are ordered by the presence of Cer. Moreover, Cer's chain ordering is greater than that of POPC in the liquid crystalline phase. However, there is no evidence of liquid-liquid phase separation in the liquid crystalline region of the POPC/Cer phase diagram.

AB - Biological membranes contain domains having distinct physical properties. We study defined mixtures of phosphoglycerolipids and sphingolipids to ascertain the fundamental interactions governing these lipids in the absence of other cell membrane components. By using 2H-NMR we have determined the temperature and composition dependencies of membrane structure and phase behavior for aqueous dispersions of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and the ceramide (Cer) N-palmitoyl-sphingosine. It is found that gel and liquid-crystalline phases coexist over a wide range of temperature and composition. Domains of different composition and phase state are present in POPC/Cer membranes at physiological temperature for Cer concentrations exceeding 15 mol %. The acyl chains of liquid crystalline phase POPC are ordered by the presence of Cer. Moreover, Cer's chain ordering is greater than that of POPC in the liquid crystalline phase. However, there is no evidence of liquid-liquid phase separation in the liquid crystalline region of the POPC/Cer phase diagram.

UR - http://www.scopus.com/inward/record.url?scp=0036109677&partnerID=8YFLogxK

U2 - 10.1016/S0006-3495(02)75650-6

DO - 10.1016/S0006-3495(02)75650-6

M3 - 期刊論文

C2 - 12023232

AN - SCOPUS:0036109677

SN - 0006-3495

VL - 82

SP - 3089

EP - 3095

JO - Biophysical Journal

JF - Biophysical Journal

IS - 6

ER -

The effect of ceramide on phosphatidylcholine membranes: A deuterium NMR study

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