The apoptotic effect of green tea (-)-epigallocatechin gallate on 3T3-L1 preadipocytes depends on the Cdk2 pathway

Bo Tsung Wu; Pei Fang Hung; Hui Chian Chen; Rong Nan Huang; Hsin Huei Chang; Yung Hsi Kao

doi:10.1021/jf050045p

The apoptotic effect of green tea (-)-epigallocatechin gallate on 3T3-L1 preadipocytes depends on the Cdk2 pathway

Bo Tsung Wu, Pei Fang Hung, Hui Chian Chen, Rong Nan Huang, Hsin Huei Chang, Yung Hsi Kao

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

74 Scopus citations

Abstract

This study was designed to investigate the effect of green tea catechins, especially (-)-epigallocatechin gallate (EGCG), on the apoptosis of 3T3-L1 preadipocytes. Preadipocyte apoptosis as indicated by formation of DNA fragments was induced by EGCG in dose-dependent manners. While EGCG was demonstrated to decrease Cdk2 expression and activity and increase caspase-3 activity, overexpression of Cdk2 and treatment with the caspase-3 inhibitor respectively prevented preadipocytes from induction of DNA fragmentation and caspase-3 activity by doses of 100-400 μM of EGCG. This suggests the Cdk2- and caspase-3-dependent apoptotic effects of EGCG. Moreover, EGCG was more effective than EC, ECG, and EGC in changing the apoptotic signals. Results of this study may relate to the mechanism by which EGCG modulates body weight.

Original language	English
Pages (from-to)	5695-5701
Number of pages	7
Journal	Journal of Agricultural and Food Chemistry
Volume	53
Issue number	14
DOIs	https://doi.org/10.1021/jf050045p
State	Published - 13 Jul 2005

Keywords

3T3-L1 preadipocyte
Apoptosis
Caspase-3
Cyclin-dependent kinase
Epigallocatechin gallate
Green tea

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10.1021/jf050045p

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title = "The apoptotic effect of green tea (-)-epigallocatechin gallate on 3T3-L1 preadipocytes depends on the Cdk2 pathway",

abstract = "This study was designed to investigate the effect of green tea catechins, especially (-)-epigallocatechin gallate (EGCG), on the apoptosis of 3T3-L1 preadipocytes. Preadipocyte apoptosis as indicated by formation of DNA fragments was induced by EGCG in dose-dependent manners. While EGCG was demonstrated to decrease Cdk2 expression and activity and increase caspase-3 activity, overexpression of Cdk2 and treatment with the caspase-3 inhibitor respectively prevented preadipocytes from induction of DNA fragmentation and caspase-3 activity by doses of 100-400 μM of EGCG. This suggests the Cdk2- and caspase-3-dependent apoptotic effects of EGCG. Moreover, EGCG was more effective than EC, ECG, and EGC in changing the apoptotic signals. Results of this study may relate to the mechanism by which EGCG modulates body weight.",

keywords = "3T3-L1 preadipocyte, Apoptosis, Caspase-3, Cyclin-dependent kinase, Epigallocatechin gallate, Green tea",

author = "Wu, {Bo Tsung} and Hung, {Pei Fang} and Chen, {Hui Chian} and Huang, {Rong Nan} and Chang, {Hsin Huei} and Kao, {Yung Hsi}",

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T1 - The apoptotic effect of green tea (-)-epigallocatechin gallate on 3T3-L1 preadipocytes depends on the Cdk2 pathway

AU - Wu, Bo Tsung

AU - Hung, Pei Fang

AU - Chen, Hui Chian

AU - Huang, Rong Nan

AU - Chang, Hsin Huei

AU - Kao, Yung Hsi

PY - 2005/7/13

Y1 - 2005/7/13

N2 - This study was designed to investigate the effect of green tea catechins, especially (-)-epigallocatechin gallate (EGCG), on the apoptosis of 3T3-L1 preadipocytes. Preadipocyte apoptosis as indicated by formation of DNA fragments was induced by EGCG in dose-dependent manners. While EGCG was demonstrated to decrease Cdk2 expression and activity and increase caspase-3 activity, overexpression of Cdk2 and treatment with the caspase-3 inhibitor respectively prevented preadipocytes from induction of DNA fragmentation and caspase-3 activity by doses of 100-400 μM of EGCG. This suggests the Cdk2- and caspase-3-dependent apoptotic effects of EGCG. Moreover, EGCG was more effective than EC, ECG, and EGC in changing the apoptotic signals. Results of this study may relate to the mechanism by which EGCG modulates body weight.

AB - This study was designed to investigate the effect of green tea catechins, especially (-)-epigallocatechin gallate (EGCG), on the apoptosis of 3T3-L1 preadipocytes. Preadipocyte apoptosis as indicated by formation of DNA fragments was induced by EGCG in dose-dependent manners. While EGCG was demonstrated to decrease Cdk2 expression and activity and increase caspase-3 activity, overexpression of Cdk2 and treatment with the caspase-3 inhibitor respectively prevented preadipocytes from induction of DNA fragmentation and caspase-3 activity by doses of 100-400 μM of EGCG. This suggests the Cdk2- and caspase-3-dependent apoptotic effects of EGCG. Moreover, EGCG was more effective than EC, ECG, and EGC in changing the apoptotic signals. Results of this study may relate to the mechanism by which EGCG modulates body weight.

KW - 3T3-L1 preadipocyte

KW - Apoptosis

KW - Caspase-3

KW - Cyclin-dependent kinase

KW - Epigallocatechin gallate

KW - Green tea

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U2 - 10.1021/jf050045p

DO - 10.1021/jf050045p

M3 - 期刊論文

C2 - 15998135

AN - SCOPUS:22544440296

SN - 0021-8561

VL - 53

SP - 5695

EP - 5701

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 14

ER -

The apoptotic effect of green tea (-)-epigallocatechin gallate on 3T3-L1 preadipocytes depends on the Cdk2 pathway

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Keywords

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