The apoptotic effect of green tea (-)-epigallocatechin gallate on 3T3-L1 preadipocytes depends on the Cdk2 pathway

Bo Tsung Wu, Pei Fang Hung, Hui Chian Chen, Rong Nan Huang, Hsin Huei Chang, Yung Hsi Kao

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

This study was designed to investigate the effect of green tea catechins, especially (-)-epigallocatechin gallate (EGCG), on the apoptosis of 3T3-L1 preadipocytes. Preadipocyte apoptosis as indicated by formation of DNA fragments was induced by EGCG in dose-dependent manners. While EGCG was demonstrated to decrease Cdk2 expression and activity and increase caspase-3 activity, overexpression of Cdk2 and treatment with the caspase-3 inhibitor respectively prevented preadipocytes from induction of DNA fragmentation and caspase-3 activity by doses of 100-400 μM of EGCG. This suggests the Cdk2- and caspase-3-dependent apoptotic effects of EGCG. Moreover, EGCG was more effective than EC, ECG, and EGC in changing the apoptotic signals. Results of this study may relate to the mechanism by which EGCG modulates body weight.

Original languageEnglish
Pages (from-to)5695-5701
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume53
Issue number14
DOIs
StatePublished - 13 Jul 2005

Keywords

  • 3T3-L1 preadipocyte
  • Apoptosis
  • Caspase-3
  • Cyclin-dependent kinase
  • Epigallocatechin gallate
  • Green tea

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