Temporal transcription program of recombinant Autographa californica multiple nucleopolyhedrosis virus

Shih Sheng Jiang, I. Shou Chang, Lin Wei Huang, Po Cheng Chen, Chi Chung Wen, Shu Chen Liu, Li Chu Chien, Chung Yen Lin, Chao A. Hsiung, Jyh Lyh Juang

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Baculoviruses, a family of large, rod-shaped viruses that mainly infect lepidopteran insects, have been widely used to transduce various cells for exogenous gene expression. Nonetheless, how a virus controls its transcription program in cells is poorly understood. With a custom-made baculovirus DNA microarray, we investigated the recombinant Autographa californica multiple nucleopolyhedrosis virus (AcMNPV) gene expression program in lepidopteran Sf21 cells over the time course of infection. Our analysis of transcription kinetics in the cells uncovered sequential viral gene expression patterns possibly regulated by different mechanisms during different phases of infection. To gain further insight into the regulatory network, we investigated the transcription program of a mutant virus deficient in an early transactivator (pe38) and uncovered several pe38-dependent and pe38-independent genes. This study of baculovirus dynamic transcription programs in different virus genetic backgrounds provides new molecular insights into how gene expression in viruses is regulated.

Original languageEnglish
Pages (from-to)8989-8999
Number of pages11
JournalJournal of Virology
Volume80
Issue number18
DOIs
StatePublished - Sep 2006

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