Temperature effect on nervous necrosis virus infection in grouper cell line and in grouper larvae

Shau Chi Chi, Su Ching Lin, Huei Meei Su, Wei Wen Hu

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54 Scopus citations


This preliminary study elucidates the in vitro and in vivo effects of temperature on grouper nervous necrosis virus (GNNV) infection. A novel continuous cell line derived from the fin tissue of a grouper (Epinephelus coioides, Hamilton), named as GF-1 cell line, was used. Cytopathic effect was observed in GNNV-infected GF-1 cells incubated at 24-32°C after viral adsorption, but not at 20°C or 37°C even though the viral adsorption temperature was 28°C. Viral protein could be detected in the pellets of GNNV-infected GF-1 cells cultured at 20-32°C, but not at 37°C. In a challenge test, GNNV-challenged larvae which were maintained at a constant 28°C began to die 1 day post challenge (p.c.) with a death rate of 80%. Mortality reached 100% by 50 h p.c., while the mortality of negative control fish was only 5%. The cumulative mortality of GNNV-challenged larvae at ambient temperature, i.e. 28°C at noon and 24°C at midnight, was 10% 1 day p.c., and increased to 100% by 80 h p.c. Based on the results, we concluded that temperature plays an important role in GNNV infection and pathogenicity. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)107-114
Number of pages8
JournalVirus Research
Issue number1-2
StatePublished - Sep 1999


  • Fish nodavirus
  • Grouper
  • Nervous necrosis virus
  • Temperature effect


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