Systems biology approach to exploring the effect of cyclic stretching on cardiac cell physiology

Chien Cheng Chen, Tzyy Yue Wong, Tzu Yun Chin, Wen Hsien Lee, Chan Yen Kuo, Yi Chiung Hsu

Research output: Contribution to journalArticlepeer-review


Although mechanical forces are involved in pressure-overloaded cardiomyopathy, their effects on gene transcription profiles are not fully understood. Here, we used next-generation sequencing (NGS) to investigate changes in genomic profiles after cyclic mechanical stretching of human cardiomyocytes. We found that 85, 87, 32, 29, and 28 genes were differentially expressed after 1, 4, 12, 24, and 48 hours of stretching. Furthermore, 10 of the 29 genes that were up-regulated and 11 of the 28 that were down-regulated after 24 h showed the same changes after 48 h. We then examined expression of the genes that encode serpin family E member 1 (SERPINE1), DNA-binding protein inhibitor 1 (ID1), DNA-binding protein inhibitor 3 (ID3), and CCL2, a cytokine that acts as chemotactic factor in monocytes, in an RT-PCR experiment. The same changes were observed for all four genes after all cyclic stretching durations, confirming the NGS results. Taken together, these findings suggest that cyclical stretching can alter cardiac cell physiology by activating cardiac cell metabolism and impacting cholesterol biosynthesis signaling.

Original languageEnglish
Pages (from-to)16035-16045
Number of pages11
Issue number16
StatePublished - 31 Aug 2020


  • Cardiac cell
  • Cyclic stretching
  • Functional enrichment
  • Next-generation sequencing


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