Surface plasmon resonance biosensor for microalbumin detection

Jen Tsai Liu, Po Shen Lin, Yue Ming Hsin, Jang Zern Tsai, Wen Yih Chen

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Human serum albumin (HSA) is an important biomarker for diagnosing nephropathy. Traditional microalbumin detection is easy affected by the intake of certain medicines, such as aspirin, corticosteroids and antibiotics. In this research, the surface plasmon resonance (SPR) biosensor technique was used to detect HSA. The anthraquinone dye Cibacron Blue F3G-A (CB) is used as a receptor which has a specific affinity for HSA. The purpose of this study is establishing the optimum conditions for SPR to detect HSA. The results exhibited that the sensor chip has high affinity as well as high capacity at pH 4 with 0.05. M sodium chloride. In addition, linear response appeared in the range of 0.01-0.1. mg/ml HSA and the detecting limit was 4μg/ml. This methodology may prove to be useful in clinical diagnosis and may serve as an assay for HSA binding ability.

Original languageEnglish
Pages (from-to)696-700
Number of pages5
JournalJournal of the Taiwan Institute of Chemical Engineers
Volume42
Issue number5
DOIs
StatePublished - Sep 2011

Keywords

  • Cibacron Blue F3G-A
  • Human serum albumin
  • Surface plasmon resonance

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