Surface plasmon resonance biosensor for microalbumin detection

Jen Tsai Liu; Po Shen Lin; Yue Ming Hsin; Jang Zern Tsai; Wen Yih Chen

doi:10.1016/j.jtice.2011.01.005

Surface plasmon resonance biosensor for microalbumin detection

Jen Tsai Liu, Po Shen Lin, Yue Ming Hsin, Jang Zern Tsai, Wen Yih Chen

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Human serum albumin (HSA) is an important biomarker for diagnosing nephropathy. Traditional microalbumin detection is easy affected by the intake of certain medicines, such as aspirin, corticosteroids and antibiotics. In this research, the surface plasmon resonance (SPR) biosensor technique was used to detect HSA. The anthraquinone dye Cibacron Blue F3G-A (CB) is used as a receptor which has a specific affinity for HSA. The purpose of this study is establishing the optimum conditions for SPR to detect HSA. The results exhibited that the sensor chip has high affinity as well as high capacity at pH 4 with 0.05. M sodium chloride. In addition, linear response appeared in the range of 0.01-0.1. mg/ml HSA and the detecting limit was 4μg/ml. This methodology may prove to be useful in clinical diagnosis and may serve as an assay for HSA binding ability.

Original language	English
Pages (from-to)	696-700
Number of pages	5
Journal	Journal of the Taiwan Institute of Chemical Engineers
Volume	42
Issue number	5
DOIs	https://doi.org/10.1016/j.jtice.2011.01.005
State	Published - Sep 2011

Keywords

Cibacron Blue F3G-A
Human serum albumin
Surface plasmon resonance

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jtice.2011.01.005

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title = "Surface plasmon resonance biosensor for microalbumin detection",

abstract = "Human serum albumin (HSA) is an important biomarker for diagnosing nephropathy. Traditional microalbumin detection is easy affected by the intake of certain medicines, such as aspirin, corticosteroids and antibiotics. In this research, the surface plasmon resonance (SPR) biosensor technique was used to detect HSA. The anthraquinone dye Cibacron Blue F3G-A (CB) is used as a receptor which has a specific affinity for HSA. The purpose of this study is establishing the optimum conditions for SPR to detect HSA. The results exhibited that the sensor chip has high affinity as well as high capacity at pH 4 with 0.05. M sodium chloride. In addition, linear response appeared in the range of 0.01-0.1. mg/ml HSA and the detecting limit was 4μg/ml. This methodology may prove to be useful in clinical diagnosis and may serve as an assay for HSA binding ability.",

keywords = "Cibacron Blue F3G-A, Human serum albumin, Surface plasmon resonance",

author = "Liu, {Jen Tsai} and Lin, {Po Shen} and Hsin, {Yue Ming} and Tsai, {Jang Zern} and Chen, {Wen Yih}",

note = "Funding Information: This study was supported by the National Science Council, Taiwan (NSC 96-2218-E-008-022).",

year = "2011",

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doi = "10.1016/j.jtice.2011.01.005",

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TY - JOUR

T1 - Surface plasmon resonance biosensor for microalbumin detection

AU - Liu, Jen Tsai

AU - Lin, Po Shen

AU - Hsin, Yue Ming

AU - Tsai, Jang Zern

AU - Chen, Wen Yih

N1 - Funding Information: This study was supported by the National Science Council, Taiwan (NSC 96-2218-E-008-022).

PY - 2011/9

Y1 - 2011/9

N2 - Human serum albumin (HSA) is an important biomarker for diagnosing nephropathy. Traditional microalbumin detection is easy affected by the intake of certain medicines, such as aspirin, corticosteroids and antibiotics. In this research, the surface plasmon resonance (SPR) biosensor technique was used to detect HSA. The anthraquinone dye Cibacron Blue F3G-A (CB) is used as a receptor which has a specific affinity for HSA. The purpose of this study is establishing the optimum conditions for SPR to detect HSA. The results exhibited that the sensor chip has high affinity as well as high capacity at pH 4 with 0.05. M sodium chloride. In addition, linear response appeared in the range of 0.01-0.1. mg/ml HSA and the detecting limit was 4μg/ml. This methodology may prove to be useful in clinical diagnosis and may serve as an assay for HSA binding ability.

AB - Human serum albumin (HSA) is an important biomarker for diagnosing nephropathy. Traditional microalbumin detection is easy affected by the intake of certain medicines, such as aspirin, corticosteroids and antibiotics. In this research, the surface plasmon resonance (SPR) biosensor technique was used to detect HSA. The anthraquinone dye Cibacron Blue F3G-A (CB) is used as a receptor which has a specific affinity for HSA. The purpose of this study is establishing the optimum conditions for SPR to detect HSA. The results exhibited that the sensor chip has high affinity as well as high capacity at pH 4 with 0.05. M sodium chloride. In addition, linear response appeared in the range of 0.01-0.1. mg/ml HSA and the detecting limit was 4μg/ml. This methodology may prove to be useful in clinical diagnosis and may serve as an assay for HSA binding ability.

KW - Cibacron Blue F3G-A

KW - Human serum albumin

KW - Surface plasmon resonance

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U2 - 10.1016/j.jtice.2011.01.005

DO - 10.1016/j.jtice.2011.01.005

M3 - 期刊論文

AN - SCOPUS:80052962445

SN - 1876-1070

VL - 42

SP - 696

EP - 700

JO - Journal of the Taiwan Institute of Chemical Engineers

JF - Journal of the Taiwan Institute of Chemical Engineers

IS - 5

ER -

Surface plasmon resonance biosensor for microalbumin detection

Abstract

Keywords

UN SDGs

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