Sugar starvation- and GA-inducible calcium-dependent protein kinase 1 feedback regulates GA biosynthesis and activates a 14-3-3 protein to confer drought tolerance in rice seedlings

Shin Lon Ho, Li Fen Huang, Chung An Lu, Siou Luan He, Chun Chin Wang, Sheng Ping Yu, Jychian Chen, Su May Yu

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

Germination followed by seedling growth constitutes two essential steps in the initiation of a new life cycle in plants, and in cereals, completion of these steps is regulated by sugar starvation and the hormone gibberellin. A calcium-dependent protein kinase 1 gene (OsCDPK1) was identified by differential screening of a cDNA library derived from sucrose-starved rice suspension cells. The expression of OsCDPK1 was found to be specifically activated by sucrose starvation among several stress conditions tested as well as activated transiently during post-germination seedling growth. In gain- and loss-of-function studies performed with transgenic rice overexpressing a constitutively active or RNA interference gene knockdown construct, respectively, OsCDPK1 was found to negatively regulate the expression of enzymes essential for GA biosynthesis. In contrast, OsCDPK1 activated the expression of a 14-3-3 protein, GF14c. Overexpression of either constitutively active OsCDPK1 or GF14c enhanced drought tolerance in transgenic rice seedlings. Hence, our studies demonstrated that OsCDPK1 transduces the post-germination Ca2+ signal derived from sugar starvation and GA, refines the endogenous GA concentration and prevents drought stress injury, all essential functions to seedling development at the beginning of the life cycle in rice.

Original languageEnglish
Pages (from-to)347-361
Number of pages15
JournalPlant Molecular Biology
Volume81
Issue number4-5
DOIs
StatePublished - Mar 2013

Keywords

  • Calcium-dependent protein kinase 1
  • Drought stress tolerance
  • GA homeostasis
  • GF14c
  • Oryza sativa

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