Studies on isolation and properties of two isoforms of pituitary gonadotropin from black silver carp, Aristichthys nobilis

Partha P. Banerjee, Subhadra Banerjee, San Tai Shen, Yung Hsi Kao, John Yuh Lin Yu

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

1. 1. Two isoforms of gonadotropins (GTHs) were isolated from pituitaries of black silver carp (Aristichthys nobilis Richardson): 37K-bscGTH and 43K-bscGTH. They differed in molecular weight, isoelectric point, and sialic acid content. 2. 2. Amino acid compositions of the respective α and β subunits were similar and the first 10 N-terminal amino acid sequences of the respective α and β subunits of the two GTH isoforms were identical. 3. 3. Both GTHs were similarly potent in stimulating loach ovulation in vivo, and common carp testicular testosterone formation and ovarian follicle estradiol-17β formation in vitro. 4. 4. Preparation of antisera of both GTHs and studies of their crossreactions with various piscine GTHs were reported for the first time.

Original languageEnglish
Pages (from-to)241-253
Number of pages13
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume104
Issue number2
DOIs
StatePublished - Feb 1993

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