Stem cell culture on polyvinyl alcohol hydrogels having different elasticity and immobilized with ECM-derived oligopeptides

Saradaprasan Muduli; Li Hua Chen; Meng Pei Li; Zhao Wen Heish; Cheng Hui Liu; Suresh Kumar; Abdullah A. Alarfaj; Murugan A. Munusamy; Giovanni Benelli; Kadarkarai Murugan; Han Chow Wang; Da Chung Chen; Shih Tien Hsu; Shih Chang Chang; Akon Higuchi

doi:10.1515/polyeng-2016-0193

Stem cell culture on polyvinyl alcohol hydrogels having different elasticity and immobilized with ECM-derived oligopeptides

Saradaprasan Muduli, Li Hua Chen, Meng Pei Li, Zhao Wen Heish, Cheng Hui Liu, Suresh Kumar, Abdullah A. Alarfaj, Murugan A. Munusamy, Giovanni Benelli, Kadarkarai Murugan, Han Chow Wang, Da Chung Chen, Shih Tien Hsu, Shih Chang Chang, Akon Higuchi

Department of Chemical and Materials Engineering

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

The physical characteristics of cell culture materials, such as their elasticity, affect stem cell fate with respect to cell proliferation and differentiation. We systematically investigated the morphologies and characteristics of several stem cell types, including human amniotic-derived stem cells, human hematopoietic stem cells, human induced pluripotent stem (iPS) cells, and embryonic stem (ES) cells on poly(vinyl alcohol) (PVA) hydrogels immobilized with and without extracellular matrix-derived oligopeptide. Human ES cells did not adhere well to soft PVA hydrogels immobilized with oligovitronectin, whereas they did adhere well to PVA hydrogel dishes with elasticities greater than 15 kPa. These results indicate that biomaterials such as PVA hydrogels should be designed to possess minimum elasticity to facilitate human ES cell attachment. PVA hydrogels immobilized with and without extracellular matrix-derived oligopeptides are excellent candidates of cell culture biomaterials for investigations into how cell culture biomaterial elasticity affects stem cell culture and differentiation.

Original language	English
Pages (from-to)	647-660
Number of pages	14
Journal	Journal of Polymer Engineering
Volume	37
Issue number	7
DOIs	https://doi.org/10.1515/polyeng-2016-0193
State	Published - 1 Sep 2017

Keywords

biomaterial
cell culture
elasticity
poly(vinyl alcohol)
stem cell

Access to Document

10.1515/polyeng-2016-0193

Development of Biomaterials for Feeder-Free and Xeno-Free Culture of Human Pluripotent Stem (Es and Ips) Cells(3/3)
Akon, H. (PI)
1/08/17 → 31/07/18
Project: Research
Continuous Harvest Bioreactor System for Expansion and Differentiation of Human Es and Ips Cells Cultured on Intelligent Biomaterials Grafted with Nanosegments( I )
Akon, H. (PI)
1/08/16 → 31/07/17
Project: Research

Cite this

Muduli, S., Chen, L. H., Li, M. P., Heish, Z. W., Liu, C. H., Kumar, S., Alarfaj, A. A., Munusamy, M. A., Benelli, G., Murugan, K., Wang, H. C., Chen, D. C., Hsu, S. T., Chang, S. C., & Higuchi, A. (2017). Stem cell culture on polyvinyl alcohol hydrogels having different elasticity and immobilized with ECM-derived oligopeptides. Journal of Polymer Engineering, 37(7), 647-660. https://doi.org/10.1515/polyeng-2016-0193

@article{377cfb48738b475fb04a4f585924103a,

title = "Stem cell culture on polyvinyl alcohol hydrogels having different elasticity and immobilized with ECM-derived oligopeptides",

abstract = "The physical characteristics of cell culture materials, such as their elasticity, affect stem cell fate with respect to cell proliferation and differentiation. We systematically investigated the morphologies and characteristics of several stem cell types, including human amniotic-derived stem cells, human hematopoietic stem cells, human induced pluripotent stem (iPS) cells, and embryonic stem (ES) cells on poly(vinyl alcohol) (PVA) hydrogels immobilized with and without extracellular matrix-derived oligopeptide. Human ES cells did not adhere well to soft PVA hydrogels immobilized with oligovitronectin, whereas they did adhere well to PVA hydrogel dishes with elasticities greater than 15 kPa. These results indicate that biomaterials such as PVA hydrogels should be designed to possess minimum elasticity to facilitate human ES cell attachment. PVA hydrogels immobilized with and without extracellular matrix-derived oligopeptides are excellent candidates of cell culture biomaterials for investigations into how cell culture biomaterial elasticity affects stem cell culture and differentiation.",

keywords = "biomaterial, cell culture, elasticity, poly(vinyl alcohol), stem cell",

author = "Saradaprasan Muduli and Chen, {Li Hua} and Li, {Meng Pei} and Heish, {Zhao Wen} and Liu, {Cheng Hui} and Suresh Kumar and Alarfaj, {Abdullah A.} and Munusamy, {Murugan A.} and Giovanni Benelli and Kadarkarai Murugan and Wang, {Han Chow} and Chen, {Da Chung} and Hsu, {Shih Tien} and Chang, {Shih Chang} and Akon Higuchi",

note = "Publisher Copyright: {\textcopyright} 2017 Walter de Gruyter GmbH, Berlin/Boston 2017.",

year = "2017",

month = sep,

day = "1",

doi = "10.1515/polyeng-2016-0193",

language = "???core.languages.en_GB???",

volume = "37",

pages = "647--660",

journal = "Journal of Polymer Engineering",

issn = "0334-6447",

number = "7",

}

Muduli, S, Chen, LH, Li, MP, Heish, ZW, Liu, CH, Kumar, S, Alarfaj, AA, Munusamy, MA, Benelli, G, Murugan, K, Wang, HC, Chen, DC, Hsu, ST, Chang, SC & Higuchi, A 2017, 'Stem cell culture on polyvinyl alcohol hydrogels having different elasticity and immobilized with ECM-derived oligopeptides', Journal of Polymer Engineering, vol. 37, no. 7, pp. 647-660. https://doi.org/10.1515/polyeng-2016-0193

TY - JOUR

T1 - Stem cell culture on polyvinyl alcohol hydrogels having different elasticity and immobilized with ECM-derived oligopeptides

AU - Muduli, Saradaprasan

AU - Chen, Li Hua

AU - Li, Meng Pei

AU - Heish, Zhao Wen

AU - Liu, Cheng Hui

AU - Kumar, Suresh

AU - Alarfaj, Abdullah A.

AU - Munusamy, Murugan A.

AU - Benelli, Giovanni

AU - Murugan, Kadarkarai

AU - Wang, Han Chow

AU - Chen, Da Chung

AU - Hsu, Shih Tien

AU - Chang, Shih Chang

AU - Higuchi, Akon

PY - 2017/9/1

Y1 - 2017/9/1

N2 - The physical characteristics of cell culture materials, such as their elasticity, affect stem cell fate with respect to cell proliferation and differentiation. We systematically investigated the morphologies and characteristics of several stem cell types, including human amniotic-derived stem cells, human hematopoietic stem cells, human induced pluripotent stem (iPS) cells, and embryonic stem (ES) cells on poly(vinyl alcohol) (PVA) hydrogels immobilized with and without extracellular matrix-derived oligopeptide. Human ES cells did not adhere well to soft PVA hydrogels immobilized with oligovitronectin, whereas they did adhere well to PVA hydrogel dishes with elasticities greater than 15 kPa. These results indicate that biomaterials such as PVA hydrogels should be designed to possess minimum elasticity to facilitate human ES cell attachment. PVA hydrogels immobilized with and without extracellular matrix-derived oligopeptides are excellent candidates of cell culture biomaterials for investigations into how cell culture biomaterial elasticity affects stem cell culture and differentiation.

AB - The physical characteristics of cell culture materials, such as their elasticity, affect stem cell fate with respect to cell proliferation and differentiation. We systematically investigated the morphologies and characteristics of several stem cell types, including human amniotic-derived stem cells, human hematopoietic stem cells, human induced pluripotent stem (iPS) cells, and embryonic stem (ES) cells on poly(vinyl alcohol) (PVA) hydrogels immobilized with and without extracellular matrix-derived oligopeptide. Human ES cells did not adhere well to soft PVA hydrogels immobilized with oligovitronectin, whereas they did adhere well to PVA hydrogel dishes with elasticities greater than 15 kPa. These results indicate that biomaterials such as PVA hydrogels should be designed to possess minimum elasticity to facilitate human ES cell attachment. PVA hydrogels immobilized with and without extracellular matrix-derived oligopeptides are excellent candidates of cell culture biomaterials for investigations into how cell culture biomaterial elasticity affects stem cell culture and differentiation.

KW - biomaterial

KW - cell culture

KW - elasticity

KW - poly(vinyl alcohol)

KW - stem cell

UR - http://www.scopus.com/inward/record.url?scp=85028890032&partnerID=8YFLogxK

U2 - 10.1515/polyeng-2016-0193

DO - 10.1515/polyeng-2016-0193

M3 - 期刊論文

AN - SCOPUS:85028890032

SN - 0334-6447

VL - 37

SP - 647

EP - 660

JO - Journal of Polymer Engineering

JF - Journal of Polymer Engineering

IS - 7

ER -

Stem cell culture on polyvinyl alcohol hydrogels having different elasticity and immobilized with ECM-derived oligopeptides

Abstract

Keywords

Access to Document

Fingerprint

Projects

Development of Biomaterials for Feeder-Free and Xeno-Free Culture of Human Pluripotent Stem (Es and Ips) Cells(3/3)

Continuous Harvest Bioreactor System for Expansion and Differentiation of Human Es and Ips Cells Cultured on Intelligent Biomaterials Grafted with Nanosegments( I )

Cite this