Stem cell culture and differentiation in 3-D scaffolds

Yasuhiko Tabata, Idaszek Joanna, Akon Higuchi

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Conventional two-dimensional (2-D) cultivation are easy to utilize for human pluripotent stem (hPS) cell cultivation in standard techniques and are important for analysis or development of the signal pathways to keep pluripotent state of hPS cells cultivated on 2-D cell culture materials. However, the most efficient protocol to prepare hPS cells is the cell culture in a three dimensional (3-D) cultivation unit because huge numbers of hPS cells should be utilized in clinical treatment. Some 3-D cultivation strategies for hPS cells are considered: (a) microencapsulated cell cultivation in suspended hydrogels, (b) cell cultivation on microcarriers (MCs), (c) cell cultivation on self-aggregated spheroid [cell aggregates; embryoid bodies (EBs) and organoids], (d) cell cultivation on microfibers or nanofibers, and (e) cell cultivation in macroporous scaffolds. These cultivation ways are described in this chapter.

Original languageEnglish
Title of host publicationProgress in Molecular Biology and Translational Science
EditorsAkon Higuchi, Yinghong Zhou, Shih-Hwa Chiou
PublisherElsevier B.V.
Pages109-127
Number of pages19
ISBN (Print)9780443134111
DOIs
StatePublished - Jan 2023

Publication series

NameProgress in Molecular Biology and Translational Science
Volume199
ISSN (Print)1877-1173
ISSN (Electronic)1878-0814

Keywords

  • Biomaterial
  • Cultivation
  • Differentiation
  • Embryonic stem cells
  • Extracellular matrix
  • Induced pluripotent stem cells
  • Scaffold

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