Squalene inhibits sodium arsenite-induced sister chromatid exchanges and micronuclei in Chinese hamster ovary-K1 cells

Shyh Rong Fan; I. Ching Ho; Fenny Lai-Fun Yeoh; Chi Jen Lin; Te Chang Lee

doi:10.1016/S0165-1218(96)90058-0

Squalene inhibits sodium arsenite-induced sister chromatid exchanges and micronuclei in Chinese hamster ovary-K1 cells

Shyh Rong Fan, I. Ching Ho, Fenny Lai-Fun Yeoh, Chi Jen Lin, Te Chang Lee

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Arsenic, widely distributed throughout our environment, is a well-established human carcinogen. We report here that squalene, a natural fish oil, is a potential agent in the reduction of sodium arsenite-induced sister chromatid exchange (SCE) and micronuclei in Chinese hamster ovary (CKO-K1) cells. Squalene dose-dependently inhibited sodium arsenite-induced SCE. At the highest concentration (160 μM), squalene reduced the SCE frequency from 8.85 to 6.47 SCEs per cell which is very close to the background level (5.82 SCEs per cell). Sodium arsenite dose-dependently induces micronucli in CHO-K1 cells, and squalene at 80 μM significantly inhibits arsenite-induced micronuclei. However, squalene did not eliminate the killing effects of arsenite on the cells and only slightly decreased intracellular accumulation of arsenic.

Original language	English
Pages (from-to)	165-169
Number of pages	5
Journal	Mutation Research - Genetic Toxicology
Volume	368
Issue number	3-4
DOIs	https://doi.org/10.1016/S0165-1218(96)90058-0
State	Published - 5 Jul 1996

Keywords

Micronucleus
Sister chromatid exchange
Sodium arsenite
Squalene

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title = "Squalene inhibits sodium arsenite-induced sister chromatid exchanges and micronuclei in Chinese hamster ovary-K1 cells",

abstract = "Arsenic, widely distributed throughout our environment, is a well-established human carcinogen. We report here that squalene, a natural fish oil, is a potential agent in the reduction of sodium arsenite-induced sister chromatid exchange (SCE) and micronuclei in Chinese hamster ovary (CKO-K1) cells. Squalene dose-dependently inhibited sodium arsenite-induced SCE. At the highest concentration (160 μM), squalene reduced the SCE frequency from 8.85 to 6.47 SCEs per cell which is very close to the background level (5.82 SCEs per cell). Sodium arsenite dose-dependently induces micronucli in CHO-K1 cells, and squalene at 80 μM significantly inhibits arsenite-induced micronuclei. However, squalene did not eliminate the killing effects of arsenite on the cells and only slightly decreased intracellular accumulation of arsenic.",

keywords = "Micronucleus, Sister chromatid exchange, Sodium arsenite, Squalene",

author = "Fan, {Shyh Rong} and Ho, {I. Ching} and {Lai-Fun Yeoh}, Fenny and Lin, {Chi Jen} and Lee, {Te Chang}",

note = "Funding Information: The authors thank Dr. Cyprian V. Weaver for careful review of the manuscriptT. his work was supportedb y AcademiaS inica and Grants NSC 84-2621-B-001-003azn dN SC85-2621-B-001-00f2rozm the NationalS cienceC ouncil,R epublico f China.",

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TY - JOUR

T1 - Squalene inhibits sodium arsenite-induced sister chromatid exchanges and micronuclei in Chinese hamster ovary-K1 cells

AU - Fan, Shyh Rong

AU - Ho, I. Ching

AU - Lai-Fun Yeoh, Fenny

AU - Lin, Chi Jen

AU - Lee, Te Chang

N1 - Funding Information: The authors thank Dr. Cyprian V. Weaver for careful review of the manuscriptT. his work was supportedb y AcademiaS inica and Grants NSC 84-2621-B-001-003azn dN SC85-2621-B-001-00f2rozm the NationalS cienceC ouncil,R epublico f China.

PY - 1996/7/5

Y1 - 1996/7/5

N2 - Arsenic, widely distributed throughout our environment, is a well-established human carcinogen. We report here that squalene, a natural fish oil, is a potential agent in the reduction of sodium arsenite-induced sister chromatid exchange (SCE) and micronuclei in Chinese hamster ovary (CKO-K1) cells. Squalene dose-dependently inhibited sodium arsenite-induced SCE. At the highest concentration (160 μM), squalene reduced the SCE frequency from 8.85 to 6.47 SCEs per cell which is very close to the background level (5.82 SCEs per cell). Sodium arsenite dose-dependently induces micronucli in CHO-K1 cells, and squalene at 80 μM significantly inhibits arsenite-induced micronuclei. However, squalene did not eliminate the killing effects of arsenite on the cells and only slightly decreased intracellular accumulation of arsenic.

AB - Arsenic, widely distributed throughout our environment, is a well-established human carcinogen. We report here that squalene, a natural fish oil, is a potential agent in the reduction of sodium arsenite-induced sister chromatid exchange (SCE) and micronuclei in Chinese hamster ovary (CKO-K1) cells. Squalene dose-dependently inhibited sodium arsenite-induced SCE. At the highest concentration (160 μM), squalene reduced the SCE frequency from 8.85 to 6.47 SCEs per cell which is very close to the background level (5.82 SCEs per cell). Sodium arsenite dose-dependently induces micronucli in CHO-K1 cells, and squalene at 80 μM significantly inhibits arsenite-induced micronuclei. However, squalene did not eliminate the killing effects of arsenite on the cells and only slightly decreased intracellular accumulation of arsenic.

KW - Micronucleus

KW - Sister chromatid exchange

KW - Sodium arsenite

KW - Squalene

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U2 - 10.1016/S0165-1218(96)90058-0

DO - 10.1016/S0165-1218(96)90058-0

M3 - 期刊論文

C2 - 8692222

AN - SCOPUS:0030570534

SN - 0165-1218

VL - 368

SP - 165

EP - 169

JO - Mutation Research - Genetic Toxicology

JF - Mutation Research - Genetic Toxicology

IS - 3-4

ER -

Squalene inhibits sodium arsenite-induced sister chromatid exchanges and micronuclei in Chinese hamster ovary-K1 cells

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Keywords

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