SPANXA suppresses EMT by inhibiting c-JUN/SNAI2 signaling in lung adenocarcinoma

Yi Jing Hsiao; Kang Yi Su; Yi Chiung Hsu; Gee Chen Chang; Jin Shing Chen; Hsuan Yu Chen; Qi Sheng Hong; Shih Chun Hsu; Po Hsiang Kang; Chia Ying Hsu; Bing Ching Ho; Tsung Hui Yang; Chia Yu Wang; Yuh Shan Jou; Pan Chyr Yang; Sung Liang Yu

doi:10.18632/oncotarget.10088

SPANXA suppresses EMT by inhibiting c-JUN/SNAI2 signaling in lung adenocarcinoma

Yi Jing Hsiao, Kang Yi Su, Yi Chiung Hsu, Gee Chen Chang, Jin Shing Chen, Hsuan Yu Chen, Qi Sheng Hong, Shih Chun Hsu, Po Hsiang Kang, Chia Ying Hsu, Bing Ching Ho, Tsung Hui Yang, Chia Yu Wang, Yuh Shan Jou, Pan Chyr Yang, Sung Liang Yu

Department of Biomedical Sciences and Engineering

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

SPANXA (Sperm Protein Associated with the Nucleus on the X-chromosome, family members A1/A2) acts as a cancer-testis antigen expressed in normal testes, but dysregulated in various tumors. We found that SPANXA is highly expressed in low-invasive CL1-0 cells compared with isogenous high-invasive CL1-5 cells. SPANXA was preferably expressed in tumor tissues and associated with the prolonged survival of lung adenocarcinomas. SPANXA suppressed the invasion and metastasis of lung cancer cells in vitro and in vivo. By the expression microarray and pathway analysis, we found that the SPANXA-altered genes were enriched in the epithelial- mesenchymal transition (EMT) pathway. SPANXA reduced SNAI2 expression resulted in up-regulating E-cadherin. c-JUN acts as the positive-regulator of EMT. Silencing SPANXA increased c-JUN mRNA expression and blockage of c-JUN led to SNAI2 down-regulation. Our results clearly characterized SPANXA as an EMT inhibitor by suppressing c-JUN-SNAI2 axis in lung adenocarcinoma.

Original language	English
Pages (from-to)	44417-44429
Number of pages	13
Journal	Oncotarget
Volume	7
Issue number	28
DOIs	https://doi.org/10.18632/oncotarget.10088
State	Published - 2016

Keywords

AP-1
E-cadherin
Metastasis
SLUG
SPANX family

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title = "SPANXA suppresses EMT by inhibiting c-JUN/SNAI2 signaling in lung adenocarcinoma",

abstract = "SPANXA (Sperm Protein Associated with the Nucleus on the X-chromosome, family members A1/A2) acts as a cancer-testis antigen expressed in normal testes, but dysregulated in various tumors. We found that SPANXA is highly expressed in low-invasive CL1-0 cells compared with isogenous high-invasive CL1-5 cells. SPANXA was preferably expressed in tumor tissues and associated with the prolonged survival of lung adenocarcinomas. SPANXA suppressed the invasion and metastasis of lung cancer cells in vitro and in vivo. By the expression microarray and pathway analysis, we found that the SPANXA-altered genes were enriched in the epithelial- mesenchymal transition (EMT) pathway. SPANXA reduced SNAI2 expression resulted in up-regulating E-cadherin. c-JUN acts as the positive-regulator of EMT. Silencing SPANXA increased c-JUN mRNA expression and blockage of c-JUN led to SNAI2 down-regulation. Our results clearly characterized SPANXA as an EMT inhibitor by suppressing c-JUN-SNAI2 axis in lung adenocarcinoma.",

keywords = "AP-1, E-cadherin, Metastasis, SLUG, SPANX family",

author = "Hsiao, {Yi Jing} and Su, {Kang Yi} and Hsu, {Yi Chiung} and Chang, {Gee Chen} and Chen, {Jin Shing} and Chen, {Hsuan Yu} and Hong, {Qi Sheng} and Hsu, {Shih Chun} and Kang, {Po Hsiang} and Hsu, {Chia Ying} and Ho, {Bing Ching} and Yang, {Tsung Hui} and Wang, {Chia Yu} and Jou, {Yuh Shan} and Yang, {Pan Chyr} and Yu, {Sung Liang}",

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doi = "10.18632/oncotarget.10088",

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T1 - SPANXA suppresses EMT by inhibiting c-JUN/SNAI2 signaling in lung adenocarcinoma

AU - Hsiao, Yi Jing

AU - Su, Kang Yi

AU - Hsu, Yi Chiung

AU - Chang, Gee Chen

AU - Chen, Jin Shing

AU - Chen, Hsuan Yu

AU - Hong, Qi Sheng

AU - Hsu, Shih Chun

AU - Kang, Po Hsiang

AU - Hsu, Chia Ying

AU - Ho, Bing Ching

AU - Yang, Tsung Hui

AU - Wang, Chia Yu

AU - Jou, Yuh Shan

AU - Yang, Pan Chyr

AU - Yu, Sung Liang

PY - 2016

Y1 - 2016

N2 - SPANXA (Sperm Protein Associated with the Nucleus on the X-chromosome, family members A1/A2) acts as a cancer-testis antigen expressed in normal testes, but dysregulated in various tumors. We found that SPANXA is highly expressed in low-invasive CL1-0 cells compared with isogenous high-invasive CL1-5 cells. SPANXA was preferably expressed in tumor tissues and associated with the prolonged survival of lung adenocarcinomas. SPANXA suppressed the invasion and metastasis of lung cancer cells in vitro and in vivo. By the expression microarray and pathway analysis, we found that the SPANXA-altered genes were enriched in the epithelial- mesenchymal transition (EMT) pathway. SPANXA reduced SNAI2 expression resulted in up-regulating E-cadherin. c-JUN acts as the positive-regulator of EMT. Silencing SPANXA increased c-JUN mRNA expression and blockage of c-JUN led to SNAI2 down-regulation. Our results clearly characterized SPANXA as an EMT inhibitor by suppressing c-JUN-SNAI2 axis in lung adenocarcinoma.

AB - SPANXA (Sperm Protein Associated with the Nucleus on the X-chromosome, family members A1/A2) acts as a cancer-testis antigen expressed in normal testes, but dysregulated in various tumors. We found that SPANXA is highly expressed in low-invasive CL1-0 cells compared with isogenous high-invasive CL1-5 cells. SPANXA was preferably expressed in tumor tissues and associated with the prolonged survival of lung adenocarcinomas. SPANXA suppressed the invasion and metastasis of lung cancer cells in vitro and in vivo. By the expression microarray and pathway analysis, we found that the SPANXA-altered genes were enriched in the epithelial- mesenchymal transition (EMT) pathway. SPANXA reduced SNAI2 expression resulted in up-regulating E-cadherin. c-JUN acts as the positive-regulator of EMT. Silencing SPANXA increased c-JUN mRNA expression and blockage of c-JUN led to SNAI2 down-regulation. Our results clearly characterized SPANXA as an EMT inhibitor by suppressing c-JUN-SNAI2 axis in lung adenocarcinoma.

KW - AP-1

KW - E-cadherin

KW - Metastasis

KW - SLUG

KW - SPANX family

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SP - 44417

EP - 44429

JO - Oncotarget

JF - Oncotarget

IS - 28

ER -

SPANXA suppresses EMT by inhibiting c-JUN/SNAI2 signaling in lung adenocarcinoma

Abstract

Keywords

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