Separation of CD34+ cells from human peripheral blood through polyurethane foaming membranes

Akon Higuchi, Ayumi Iizuka, Yumiko Gomei, Toyohiko Miyazaki, Masaru Sakurai, Yuki Matsuoka, Shizue Hayashi Natori

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Cell separation from peripheral blood was investigated using polyurethane (PU) foaming membranes and PU membranes (pore size, 5 or 12 μm) at different blood permeation speeds. Permeation ratio of hematopoietic stem cells (CD34 + cells) through the PU membranes was the lowest among the blood cells at any blood permeation speed. This is thought to be because CD34 + cells are more adhesive than red blood cells (RBCs), platelets, T cells, and B cells. Primitive hematopoietic stem and progenitor cells tend to adhere to the surface of mature blood cells, because of the high expression of cell-adhesion molecules on the surface of the cells. Human serum albumin solution was exposed to PU-COOH membranes to detach adhered cells from the surface of the membranes, allowing isolation of CD34+ cells and reduction of RBCs in the permeate solution. Most purified CD34+ cells (high recovery ratio of CD34+ cells divided by recovery ratio of RBCs) were obtained in the recovery process using PU-COOH membranes (pore size, 5.2 μm) at a permeation speed of 0.3-1 mL/min.

Original languageEnglish
Pages (from-to)491-499
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume78
Issue number3
DOIs
StatePublished - 1 Sep 2006

Keywords

  • Biomaterials
  • Blood
  • CD34 cell
  • Cell separation
  • Polyurethane membrane

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