Selective separation of caseinophosphopeptides through immobilized metal ion affinity chromatography: Effects of pH values, salt concentrations and degree of phosphorylation

F. Y. Lin, W. Y. Chen, S. Y. Huang

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

A method based on immobilized metal ion affinity chromatography (IMAC) using a CS-IDA-Cu(II) gel is described for the separation of caseinophosphopeptides (CPPs) from a casein hydrolysate. According to the amino acid sequence and the degree of phosphorylation, operating parameters, such as pH values, salt concentrations for selective purification of different CPPs can be designed.

Original languageEnglish
Pages (from-to)467-471
Number of pages5
JournalBioprocess Engineering
Volume23
Issue number5
DOIs
StatePublished - 2000

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