S argassum muticum-synthesized silver nanoparticles: an effective control tool against mosquito vectors and bacterial pathogens

Pari Madhiyazhagan, Kadarkarai Murugan, Arjunan Naresh Kumar, Thiyagarajan Nataraj, Devakumar Dinesh, Chellasamy Panneerselvam, Jayapal Subramaniam, Palanisamy Mahesh Kumar, Udaiyan Suresh, Mathath Roni, Marcello Nicoletti, Abdullah A. Alarfaj, Akon Higuchi, Murugan A. Munusamy, Giovanni Benelli

Research output: Contribution to journalArticlepeer-review

97 Scopus citations

Abstract

Mosquito-borne diseases represent a deadly threat for millions of people worldwide. Furthermore, pathogens and parasites polluting water also constitute a severe plague for populations of developing countries. In this research, silver nanoparticles (AgNP) were synthesized using the aqueous extract of the seaweed Sargassum muticum. The production of AgNP was confirmed by surface plasmon resonance band illustrated in UV–vis spectrophotometry. AgNP were characterized by FTIR, SEM, EDX, and XRD analyses. AgNP were mostly spherical in shape, crystalline in nature, with face-centered cubic geometry, and mean size was 43–79 nm. Toxicity of AgNP was assessed against Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus. In laboratory, AgNP were highly toxic against larvae and pupae of the three mosquito species. Maximum efficacy was observed against A. stephensi larvae, with LC50 ranging from 16.156 ppm (larva I) to 28.881 ppm (pupa). In the field, a single treatment with AgNP (10 × LC50) in water storage reservoirs was effective against the three mosquito vectors, allowing complete elimination of larval populations after 72 h. In ovicidal experiments, egg hatchability was reduced by 100 % after treatment with 30 ppm of AgNP. Ovideterrence assays highlighted that 10 ppm of AgNP reduced oviposition rates of more than 70 % in A. aegypti, A. stephensi, and C. quinquefasciatus (OAI = −0.61, −0.63, and −0.58, respectively). Antibacterial properties of AgNP were evaluated against Bacillus subtilis, Klebsiella pneumoniae, and Salmonella typhi using the agar disk diffusion and minimum inhibitory concentration protocol. AgNP tested at 50 ppm evoked growth inhibition zones larger than 5 mm in all tested bacteria. Overall, the chance to use S. muticum-synthesized AgNP for control of mosquito vectors seems promising since they are effective at low doses and may constitute an advantageous alternative to build newer and safer mosquito control tools. This is the first report about ovicidal activity of metal nanoparticles against mosquito vectors.

Original languageEnglish
Pages (from-to)4305-4317
Number of pages13
JournalParasitology Research
Volume114
Issue number11
DOIs
StatePublished - 26 Nov 2015

Keywords

  • Aedes aegypti
  • Anopheles stephensi
  • Bacillus subtilis
  • Culex quinquefasciatus
  • Klebsiella pneumoniae
  • Mosquitocidal activity
  • Ovideterrence
  • Salmonella typhi

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