Retroconversion of estrogens into androgens by bacteria via a cobalamin-mediated methylation

Po Hsiang Wang, Yi Lung Chen, Sean Ting Shyang Wei, Kan Wu, Tzong Huei Lee, Tien Yu Wu, Yin Ru Chiang

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Steroid estrogens modulate physiology and development of vertebrates. Conversion of C19 androgens into C18 estrogens is thought to be an irreversible reaction. Here, we report a denitrifying Denitratisoma sp. strain DHT3 capable of catabolizing estrogens or androgens anaerobically. Strain DHT3 genome contains a polycistronic gene cluster, emtABCD, differentially transcribed under estrogen-fed conditions and predicted to encode a cobalamin-dependent methyltransferase system conserved among estrogen-utilizing anaerobes; an emtA-disrupted DHT3 derivative could catabolize androgens but not estrogens. These data, along with the observed androgen production in estrogen-fed strain DHT3 cultures, suggested the occurrence of a cobalamin-dependent estrogen methylation to form androgens. Consistently, the estrogen conversion into androgens in strain DHT3 cell extracts requires methylcobalamin and is inhibited by propyl iodide, a specific inhibitor of cobalamin-dependent enzymes. The identification of the cobalamin-dependent estrogen methylation thus represents an unprecedented metabolic link between cobalamin and steroid metabolism and suggests that retroconversion of estrogens into androgens occurs in the biosphere.

Original languageEnglish
Pages (from-to)1395-1403
Number of pages9
JournalProceedings of the National Academy of Sciences of the United States of America
Volume117
Issue number3
DOIs
StatePublished - 21 Jan 2020

Keywords

  • Biocatalysis
  • Cobalamin-dependent methyltransferase
  • Estrogens
  • Microbial metabolism
  • Steroids

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