Resonance-enhanced functional third harmonic optical microscopy

Shi Wei Chu, Szu Yu Chen, Tsung Han Tsai, Tzu Ming Liu, Shr Bin Wu, Sajal Biring, Juen Kai Wang, Yu Lin Wang, Kevin Chen, Bai Lin Lin, C. K. Sun

Research output: Contribution to journalConference articlepeer-review


For the purpose of functional third harmonic optical microscopy, it is necessary to find a method to locally enhance third harmonic generation at specific cellular site. We have demonstrated that by matching the third harmonic generation frequency of a Cr:forsterite laser and the surface plasmon resonance frequency of <50-nm silver nanoparticles, localized enhancement of third harmonic intensity of more than 100-folds can be achieved both in phantom and in real biological tissues. This strongly enhanced third harmonic signal can then be applied to specific molecule imaging by attaching the nanoparticles to the target molecule with the advantages of noninvasiveness and deep penetration capability.

Original languageEnglish
Pages (from-to)306-313
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - 2004
EventProgress in Biomedical Optics and Imaging - Multiphoton Microscopy in the Biomedical Sciences IV - San Jose, CA, United States
Duration: 25 Jan 200427 Jan 2004


  • Cr:forsterite laser
  • Laser scanning microscopy
  • Nanoparticles
  • Surface plasmon
  • Third-harmonic generation


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