Resonance-enhanced functional third harmonic optical microscopy

Shi Wei Chu; Szu Yu Chen; Tsung Han Tsai; Tzu Ming Liu; Shr Bin Wu; Sajal Biring; Juen Kai Wang; Yu Lin Wang; Kevin Chen; Bai Lin Lin; C. K. Sun

doi:10.1117/12.528130

Resonance-enhanced functional third harmonic optical microscopy

Shi Wei Chu, Szu Yu Chen, Tsung Han Tsai, Tzu Ming Liu, Shr Bin Wu, Sajal Biring, Juen Kai Wang, Yu Lin Wang, Kevin Chen, Bai Lin Lin, C. K. Sun

Department of Optics and Photonics

Research output: Contribution to journal › Conference article › peer-review

Abstract

For the purpose of functional third harmonic optical microscopy, it is necessary to find a method to locally enhance third harmonic generation at specific cellular site. We have demonstrated that by matching the third harmonic generation frequency of a Cr:forsterite laser and the surface plasmon resonance frequency of <50-nm silver nanoparticles, localized enhancement of third harmonic intensity of more than 100-folds can be achieved both in phantom and in real biological tissues. This strongly enhanced third harmonic signal can then be applied to specific molecule imaging by attaching the nanoparticles to the target molecule with the advantages of noninvasiveness and deep penetration capability.

Original language	English
Pages (from-to)	306-313
Number of pages	8
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	5323
DOIs	https://doi.org/10.1117/12.528130
State	Published - 2004
Event	Progress in Biomedical Optics and Imaging - Multiphoton Microscopy in the Biomedical Sciences IV - San Jose, CA, United States Duration: 25 Jan 2004 → 27 Jan 2004

Keywords

Cr:forsterite laser
Laser scanning microscopy
Nanoparticles
Surface plasmon
Third-harmonic generation

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10.1117/12.528130

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title = "Resonance-enhanced functional third harmonic optical microscopy",

abstract = "For the purpose of functional third harmonic optical microscopy, it is necessary to find a method to locally enhance third harmonic generation at specific cellular site. We have demonstrated that by matching the third harmonic generation frequency of a Cr:forsterite laser and the surface plasmon resonance frequency of <50-nm silver nanoparticles, localized enhancement of third harmonic intensity of more than 100-folds can be achieved both in phantom and in real biological tissues. This strongly enhanced third harmonic signal can then be applied to specific molecule imaging by attaching the nanoparticles to the target molecule with the advantages of noninvasiveness and deep penetration capability.",

keywords = "Cr:forsterite laser, Laser scanning microscopy, Nanoparticles, Surface plasmon, Third-harmonic generation",

author = "Chu, {Shi Wei} and Chen, {Szu Yu} and Tsai, {Tsung Han} and Liu, {Tzu Ming} and Wu, {Shr Bin} and Sajal Biring and Wang, {Juen Kai} and Wang, {Yu Lin} and Kevin Chen and Lin, {Bai Lin} and Sun, {C. K.}",

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TY - JOUR

T1 - Resonance-enhanced functional third harmonic optical microscopy

AU - Chu, Shi Wei

AU - Chen, Szu Yu

AU - Tsai, Tsung Han

AU - Liu, Tzu Ming

AU - Wu, Shr Bin

AU - Biring, Sajal

AU - Wang, Juen Kai

AU - Wang, Yu Lin

AU - Chen, Kevin

AU - Lin, Bai Lin

AU - Sun, C. K.

PY - 2004

Y1 - 2004

N2 - For the purpose of functional third harmonic optical microscopy, it is necessary to find a method to locally enhance third harmonic generation at specific cellular site. We have demonstrated that by matching the third harmonic generation frequency of a Cr:forsterite laser and the surface plasmon resonance frequency of <50-nm silver nanoparticles, localized enhancement of third harmonic intensity of more than 100-folds can be achieved both in phantom and in real biological tissues. This strongly enhanced third harmonic signal can then be applied to specific molecule imaging by attaching the nanoparticles to the target molecule with the advantages of noninvasiveness and deep penetration capability.

AB - For the purpose of functional third harmonic optical microscopy, it is necessary to find a method to locally enhance third harmonic generation at specific cellular site. We have demonstrated that by matching the third harmonic generation frequency of a Cr:forsterite laser and the surface plasmon resonance frequency of <50-nm silver nanoparticles, localized enhancement of third harmonic intensity of more than 100-folds can be achieved both in phantom and in real biological tissues. This strongly enhanced third harmonic signal can then be applied to specific molecule imaging by attaching the nanoparticles to the target molecule with the advantages of noninvasiveness and deep penetration capability.

KW - Cr:forsterite laser

KW - Laser scanning microscopy

KW - Nanoparticles

KW - Surface plasmon

KW - Third-harmonic generation

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U2 - 10.1117/12.528130

DO - 10.1117/12.528130

M3 - 會議論文

AN - SCOPUS:5644253091

SN - 0277-786X

VL - 5323

SP - 306

EP - 313

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Progress in Biomedical Optics and Imaging - Multiphoton Microscopy in the Biomedical Sciences IV

Y2 - 25 January 2004 through 27 January 2004

ER -

Resonance-enhanced functional third harmonic optical microscopy

Abstract

Keywords

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