Abstract
Control over neurite outgrowth is fundamentally important not only in biophysics and neuroscience application, but also for the preparation of neurite circuits in vitro (development of neuron-computers) as well as for in vivo nerve regeneration. The effect of neurite outgrowth of PC12 cells on collagen-coated glass plates under intermittent light irradiation at 525 nm and 0.4 mW/cm2 of intensity was investigated in this study. Neurite outgrowth of PC12 cells was significantly suppressed when PC12 cells were cultivated under intermittent light irradiation with a total irradiation time of more than 2 min per hour. No temperature increase was observed in the culture medium under either continuous or intermittent light irradiation. Therefore, suppression of neurite outgrowth under light irradiation was not due to the increase of temperature in the culture medium, but rather the effect of light on the PC12 cells; especially the signal transmittance of light to PC12 cells. The light irradiation interval also affected the neurite outgrowth of PC12 cells when the total irradiation time was constant.
| Original language | English |
|---|---|
| Pages | 2338 |
| Number of pages | 1 |
| State | Published - 2005 |
| Event | 54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: 25 May 2005 → 27 May 2005 |
Conference
| Conference | 54th SPSJ Annual Meeting 2005 |
|---|---|
| Country/Territory | Japan |
| City | Yokohama |
| Period | 25/05/05 → 27/05/05 |
Keywords
- Neurite outgrowth
- Neuron computer
- Neuronal network
- PC12 cells
- Visible light