Recent advances in protein profiling of tissues and tissue fluids

Shi Yang, Chun Ming Huang

Research output: Contribution to journalReview articlepeer-review

16 Scopus citations

Abstract

Creating protein profiles of tissues and tissue fluids, which contain secreted proteins and peptides released from various cells, is critical for biomarker discovery as well as drug and vaccine target selection. It is extremely difficult to obtain pure samples from tissues or tissue fluids, however, and identification of complex protein mixtures is still a challenge for mass spectrometry analysis. Here, we summarize recent advances in techniques for extracting proteins from tissues for mass spectrometry profiling and imaging. We also introduce a novel technique using a capillary ultrafiltration (CUF) probe to enable in vivo collection of proteins from the tissue microenvironment. The CUF probe technique is compared with existing sampling techniques, including perfusion, saline wash, fine-needle aspiration and microdialysis. In this review, we also highlight quantitative mass spectrometric proteomic approaches with, and without, stable-isotope labels. Advances in quantitative proteomics will significantly improve protein profiling of tissue and tissue fluid samples collected by CUF probes.

Original languageEnglish
Pages (from-to)515-529
Number of pages15
JournalExpert Review of Proteomics
Volume4
Issue number4
DOIs
StatePublished - Aug 2007

Keywords

  • Fluids
  • Mass spectrometry
  • Profiling
  • Protein
  • Tissue imaging
  • Ultrafiltration sampling

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