Rapid mechanochemical encapsulation of biocatalysts into robust metal–organic frameworks

Tz Han Wei, Shi Hong Wu, Yi Da Huang, Wei Shang Lo, Benjamin P. Williams, Sheng Yu Chen, Hsun Chih Yang, Yu Shen Hsu, Zih Yin Lin, Xin Hua Chen, Pei En Kuo, Lien Yang Chou, Chia Kuang Tsung, Fa Kuen Shieh

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80 Scopus citations


Metal–organic frameworks (MOFs) have recently garnered consideration as an attractive solid substrate because the highly tunable MOF framework can not only serve as an inert host but also enhance the selectivity, stability, and/or activity of the enzymes. Herein, we demonstrate the advantages of using a mechanochemical strategy to encapsulate enzymes into robust MOFs. A range of enzymes, namely β-glucosidase, invertase, β-galactosidase, and catalase, are encapsulated in ZIF-8, UiO-66-NH2, or Zn-MOF-74 via a ball milling process. The solid-state mechanochemical strategy is rapid and minimizes the use of organic solvents and strong acids during synthesis, allowing the encapsulation of enzymes into three prototypical robust MOFs while maintaining enzymatic biological activity. The activity of encapsulated enzyme is demonstrated and shows increased resistance to proteases, even under acidic conditions. This work represents a step toward the creation of a suite of biomolecule-in-MOF composites for application in a variety of industrial processes.

Original languageEnglish
Article number5002
JournalNature Communications
Issue number1
StatePublished - 1 Dec 2019


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