Quantitative and amplification-free detection of SOCS-1 CpG methylation percentage analyses in gastric cancer by fiber optic nanoplasmonic biosensor

Lakshmi Sujatha Guthula, Kun Tu Yeh, Wen Long Huang, Chun Hsien Chen, Yen Ling Chen, Chun Jen Huang, Lai Kwan Chau, Michael W.Y. Chan, Shu Hui Lin

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

A new innovative approach is essential for early and effective diagnosis of gastric cancer, using promoter hypermethylation of the tumor suppressor, SOCS-1, that is frequently inactivated in human cancers. We have developed an amplification-free fiber optic nanoplasmonic biosensor for detecting DNA methylation of the SOCS-1 human genome. The method is based on the fiber optic nanogold-linked sorbent assay of PCR-free DNA from human gastric tumor tissue and cell lines. We designed a specific DNA probe fabricated on the fiber core surface while the other probe is bioconjugated with gold nanoparticles in free form to allow percentage determination and differentiating the methylated and unmethylated cell lines, further demonstrating the SOCS-1 methylation occurs in cancer patients but not in normal cell lines. The observed detection limit is 0.81 fM for methylated DNA, and the detection time is within 15 min. In addition, our data were significantly correlated to the data obtained from PCR-based pyrosequencing, and yet with superior accuracy. Hence our results provide new insight to the quantitative evaluation of methylation status of the human genome and can act as an alternative to PCR with a great potential.

Original languageEnglish
Article number114540
JournalBiosensors and Bioelectronics
Volume214
DOIs
StatePublished - 15 Oct 2022

Keywords

  • DNA methylation
  • Fiber optic biosensor
  • Gastric cancer
  • Gold nanoparticle
  • Localized surface plasmon resonance
  • SOCS-1

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