Purification of colon carcinoma cells from primary colon tumor using a filtration method via porous polymeric filters

Jia Hua Wang, Lee Kiat Ban, Henry Hsin Chung Lee, Yen Hung Chen, Hui Yu Lin, Zhe Wei Zhu, Her Young Su, Akihiro Umezawa, Abdulrahman I. Almansour, Natarajan Arumugam, Raju Suresh Kumar, Gwo Jang Wu, Akon Higuchi

Research output: Contribution to journalArticlepeer-review


Cancer stem cells (CSCs) or cancer-initiating cells (CICs) are key factors for tumor generation and metastasis. We investigated a filtration method to enhance CSCs (CICs) from colon carcinoma HT-29 cells and primary colon carcinoma cells derived from patient colon tumors using poly(lactide-co-glycolic acid)/silk screen (PLGA/SK) filters. The colon carcinoma cell solutions were permeated via porous filters to obtain a permeation solution. Then, the cell cultivation media were permeated via the filters to obtain the recovered solution, where the colon carcinoma cells that adhered to the filters were washed off into the recovered solution. Subsequently, the filters were incubated in the culture media to obtain the migrated cells via the filters. Colon carcinoma HT-29 cells with high tumorigenicity, which might be CSCs (CICs), were enhanced in the cells in the recovered solution and in the migrated cells based on the CSC (CIC) marker expression, colony-forming unit assay, and carcinoembryonic antigen (CEA) production. Although primary colon carcinoma cells isolated from colon tumor tissues contained fibroblast-like cells, the primary colon carcinoma cells were purified from fibroblast-like cells by filtration through PLGA/SK filters, indicating that the filtration method is effective in purifying primary colon carcinoma cells.

Original languageEnglish
Article number3411
Issue number19
StatePublished - 1 Oct 2021


  • Cancer stem-like cell
  • Carcinoembryonic antigen
  • Colon carcinoma cell
  • Colony-forming unit
  • Filter
  • Filtration
  • Poly(lactide-co-glycolic acid)
  • Silk


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