Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods

Hong Reng Lin, Chao Wen Heish, Cheng Hui Liu, Saradaprasan Muduli, Hsing Fen Li, Akon Higuchi, S. Suresh Kumar, Abdullah A. Alarfaj, Murugan A. Munusamy, Shih Tien Hsu, Da Chung Chen, Giovanni Benelli, Kadarkarai Murugan, Nai Chen Cheng, Han Chow Wang, Gwo Jang Wu

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Human adipose-derived stem cells (hADSCs) are easily isolated from fat tissue without ethical concerns, but differ in purity, pluripotency, differentiation ability, and stem cell marker expression, depending on the isolation method. We isolated hADSCs from a primary fat tissue solution using: (1) conventional culture, (2) a membrane filtration method, (3) a membrane migration method where the primary cell solution was permeated through membranes, adhered hADSCs were cultured, and hADSCs migrated out from the membranes. Expression of mesenchymal stem cell markers and pluripotency genes, and osteogenic differentiation were compared for hADSCs isolated by different methods using nylon mesh filter membranes with pore sizes ranging from 11 to 80 μm. hADSCs isolated by the membrane migration method had the highest MSC surface marker expression and efficient differentiation into osteoblasts. Osteogenic differentiation ability of hADSCs and MSC surface marker expression were correlated, but osteogenic differentiation ability and pluripotent gene expression were not.

Original languageEnglish
Article number40069
JournalScientific Reports
Volume7
DOIs
StatePublished - 10 Jan 2017

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