Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods

Hong Reng Lin; Chao Wen Heish; Cheng Hui Liu; Saradaprasan Muduli; Hsing Fen Li; Akon Higuchi; S. Suresh Kumar; Abdullah A. Alarfaj; Murugan A. Munusamy; Shih Tien Hsu; Da Chung Chen; Giovanni Benelli; Kadarkarai Murugan; Nai Chen Cheng; Han Chow Wang; Gwo Jang Wu

doi:10.1038/srep40069

Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods

Hong Reng Lin, Chao Wen Heish, Cheng Hui Liu, Saradaprasan Muduli, Hsing Fen Li, Akon Higuchi, S. Suresh Kumar, Abdullah A. Alarfaj, Murugan A. Munusamy, Shih Tien Hsu, Da Chung Chen, Giovanni Benelli, Kadarkarai Murugan, Nai Chen Cheng, Han Chow Wang, Gwo Jang Wu

Department of Chemical and Materials Engineering

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Human adipose-derived stem cells (hADSCs) are easily isolated from fat tissue without ethical concerns, but differ in purity, pluripotency, differentiation ability, and stem cell marker expression, depending on the isolation method. We isolated hADSCs from a primary fat tissue solution using: (1) conventional culture, (2) a membrane filtration method, (3) a membrane migration method where the primary cell solution was permeated through membranes, adhered hADSCs were cultured, and hADSCs migrated out from the membranes. Expression of mesenchymal stem cell markers and pluripotency genes, and osteogenic differentiation were compared for hADSCs isolated by different methods using nylon mesh filter membranes with pore sizes ranging from 11 to 80 μm. hADSCs isolated by the membrane migration method had the highest MSC surface marker expression and efficient differentiation into osteoblasts. Osteogenic differentiation ability of hADSCs and MSC surface marker expression were correlated, but osteogenic differentiation ability and pluripotent gene expression were not.

Original language	English
Article number	40069
Journal	Scientific Reports
Volume	7
DOIs	https://doi.org/10.1038/srep40069
State	Published - 10 Jan 2017

Access to Document

10.1038/srep40069

Continuous Harvest Bioreactor System for Expansion and Differentiation of Human Es and Ips Cells Cultured on Intelligent Biomaterials Grafted with Nanosegments( I )
Akon, H. (PI)
1/08/16 → 31/07/17
Project: Research
Development of Biomaterials for Feeder-Free and Xeno-Free Culture of Human Pluripotent Stem (Es and Ips) Cells(2/3)
Akon, H. (PI)
1/08/16 → 31/07/17
Project: Research

Cite this

Lin, H. R., Heish, C. W., Liu, C. H., Muduli, S., Li, H. F., Higuchi, A., Kumar, S. S., Alarfaj, A. A., Munusamy, M. A., Hsu, S. T., Chen, D. C., Benelli, G., Murugan, K., Cheng, N. C., Wang, H. C., & Wu, G. J. (2017). Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods. Scientific Reports, 7, Article 40069. https://doi.org/10.1038/srep40069

@article{a260f6c9de544e5ca58841e26c35099c,

title = "Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods",

abstract = "Human adipose-derived stem cells (hADSCs) are easily isolated from fat tissue without ethical concerns, but differ in purity, pluripotency, differentiation ability, and stem cell marker expression, depending on the isolation method. We isolated hADSCs from a primary fat tissue solution using: (1) conventional culture, (2) a membrane filtration method, (3) a membrane migration method where the primary cell solution was permeated through membranes, adhered hADSCs were cultured, and hADSCs migrated out from the membranes. Expression of mesenchymal stem cell markers and pluripotency genes, and osteogenic differentiation were compared for hADSCs isolated by different methods using nylon mesh filter membranes with pore sizes ranging from 11 to 80 μm. hADSCs isolated by the membrane migration method had the highest MSC surface marker expression and efficient differentiation into osteoblasts. Osteogenic differentiation ability of hADSCs and MSC surface marker expression were correlated, but osteogenic differentiation ability and pluripotent gene expression were not.",

author = "Lin, {Hong Reng} and Heish, {Chao Wen} and Liu, {Cheng Hui} and Saradaprasan Muduli and Li, {Hsing Fen} and Akon Higuchi and Kumar, {S. Suresh} and Alarfaj, {Abdullah A.} and Munusamy, {Murugan A.} and Hsu, {Shih Tien} and Chen, {Da Chung} and Giovanni Benelli and Kadarkarai Murugan and Cheng, {Nai Chen} and Wang, {Han Chow} and Wu, {Gwo Jang}",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2017.",

year = "2017",

month = jan,

day = "10",

doi = "10.1038/srep40069",

language = "???core.languages.en_GB???",

volume = "7",

journal = "Scientific Reports",

issn = "2045-2322",

}

Lin, HR, Heish, CW, Liu, CH, Muduli, S, Li, HF, Higuchi, A, Kumar, SS, Alarfaj, AA, Munusamy, MA, Hsu, ST, Chen, DC, Benelli, G, Murugan, K, Cheng, NC, Wang, HC & Wu, GJ 2017, 'Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods', Scientific Reports, vol. 7, 40069. https://doi.org/10.1038/srep40069

TY - JOUR

T1 - Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods

AU - Lin, Hong Reng

AU - Heish, Chao Wen

AU - Liu, Cheng Hui

AU - Muduli, Saradaprasan

AU - Li, Hsing Fen

AU - Higuchi, Akon

AU - Kumar, S. Suresh

AU - Alarfaj, Abdullah A.

AU - Munusamy, Murugan A.

AU - Hsu, Shih Tien

AU - Chen, Da Chung

AU - Benelli, Giovanni

AU - Murugan, Kadarkarai

AU - Cheng, Nai Chen

AU - Wang, Han Chow

AU - Wu, Gwo Jang

PY - 2017/1/10

Y1 - 2017/1/10

N2 - Human adipose-derived stem cells (hADSCs) are easily isolated from fat tissue without ethical concerns, but differ in purity, pluripotency, differentiation ability, and stem cell marker expression, depending on the isolation method. We isolated hADSCs from a primary fat tissue solution using: (1) conventional culture, (2) a membrane filtration method, (3) a membrane migration method where the primary cell solution was permeated through membranes, adhered hADSCs were cultured, and hADSCs migrated out from the membranes. Expression of mesenchymal stem cell markers and pluripotency genes, and osteogenic differentiation were compared for hADSCs isolated by different methods using nylon mesh filter membranes with pore sizes ranging from 11 to 80 μm. hADSCs isolated by the membrane migration method had the highest MSC surface marker expression and efficient differentiation into osteoblasts. Osteogenic differentiation ability of hADSCs and MSC surface marker expression were correlated, but osteogenic differentiation ability and pluripotent gene expression were not.

AB - Human adipose-derived stem cells (hADSCs) are easily isolated from fat tissue without ethical concerns, but differ in purity, pluripotency, differentiation ability, and stem cell marker expression, depending on the isolation method. We isolated hADSCs from a primary fat tissue solution using: (1) conventional culture, (2) a membrane filtration method, (3) a membrane migration method where the primary cell solution was permeated through membranes, adhered hADSCs were cultured, and hADSCs migrated out from the membranes. Expression of mesenchymal stem cell markers and pluripotency genes, and osteogenic differentiation were compared for hADSCs isolated by different methods using nylon mesh filter membranes with pore sizes ranging from 11 to 80 μm. hADSCs isolated by the membrane migration method had the highest MSC surface marker expression and efficient differentiation into osteoblasts. Osteogenic differentiation ability of hADSCs and MSC surface marker expression were correlated, but osteogenic differentiation ability and pluripotent gene expression were not.

UR - http://www.scopus.com/inward/record.url?scp=85009165776&partnerID=8YFLogxK

U2 - 10.1038/srep40069

DO - 10.1038/srep40069

M3 - 期刊論文

C2 - 28071738

AN - SCOPUS:85009165776

SN - 2045-2322

VL - 7

JO - Scientific Reports

JF - Scientific Reports

M1 - 40069

ER -

Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods

Abstract

Access to Document

Fingerprint

Projects

Continuous Harvest Bioreactor System for Expansion and Differentiation of Human Es and Ips Cells Cultured on Intelligent Biomaterials Grafted with Nanosegments( I )

Development of Biomaterials for Feeder-Free and Xeno-Free Culture of Human Pluripotent Stem (Es and Ips) Cells(2/3)

Cite this