Protocols in stem cell culture

Tong Jiabei, Thilakavathy Karuppiah, Sun Zhong, Akon Higuchi, Suresh Kumar Subbiah

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Adult stem cells are undifferentiated cells found within differentiated tissues that possess the unique ability to renew themselves and differentiate into specialized tissue cell types. Mature stem cells can exist in different tissues and organs of the body. Under certain conditions, these cells can form new stem cells or differentiate following specific procedures to form adult pluripotent stem cells (APSCs), thereby maintaining the dynamic balance of growth and aging in tissues and organs. Adult stem cells in individual adult tissues primarily exist in a dormant state under normal conditions. However, they may manifest different levels of regeneration and renewal under pathological conditions or when induced by external stimuli. Adult stem cells display several advantages in transplant therapy due to (1) their abundant supply; (2) their property to evade immune rejection and immunosuppressive agents such as histocompatibility factor is obtained from the patients themselves; (3) their low risk of carcinogenesis with little ethical concerns; and (4) their potential of multidirectional differentiation.

Original languageEnglish
Title of host publicationStem Cell Laboratory Techniques
Subtitle of host publicationA Guide for Researchers and Students
Number of pages29
ISBN (Electronic)9780128237298
ISBN (Print)9780128237304
StatePublished - 1 Jan 2023


  • Biomaterials
  • Bone marrow
  • Cell cryopreservation
  • Cell culture
  • Protocols
  • Stem cells


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