Protection from oxidative damage using Bidens pilosa extracts in normal human erythrocytes

Hsin Ling Yang, Ssu Ching Chen, Nai Wen Chang, Jia Ming Chang, Mei Ling Lee, Pei Chuan Tsai, Han Hsuan Fu, Wei Wan Kao, Hsiao Chi Chiang, Hsuan Hui Wang, You Cheng Hseu

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103 Scopus citations


Bidens pilosa (B. pilosa) is well known in Taiwan as a traditional Chinese medicine. The purpose of this study was to evaluate the ability of both the ethanol (EtOH) and ethylacetate/ethanol (EA/EtOH) extracts from the whole B. pilosa plant, to protect normal human erythrocytes against oxidative damage in vitro. It was determined that the oxidative hemolysis and lipid/protein peroxidation of erythrocytes induced by the aqueous peroxyl radical [2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)] were suppressed by both EtOH (50-150 μg/ml) and EA/EtOH (25-75 μg/ml) extracts of B. pilosa in concentration- and time-dependent manners. B. pilosa extracts also prevented the decline of superoxide dismutase (SOD) activity and the depletion of cytosolic glutathione (GSH) and ATP in erythrocytes. These results imply that B. pilosa may have protective antioxidant properties.

Original languageEnglish
Pages (from-to)1513-1521
Number of pages9
JournalFood and Chemical Toxicology
Issue number9
StatePublished - Sep 2006


  • Antioxidants
  • Bidens pilosa
  • Glutathione
  • Hemolysis
  • Superoxide dismutase


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