Protection from oxidative damage using Bidens pilosa extracts in normal human erythrocytes

Hsin Ling Yang; Ssu Ching Chen; Nai Wen Chang; Jia Ming Chang; Mei Ling Lee; Pei Chuan Tsai; Han Hsuan Fu; Wei Wan Kao; Hsiao Chi Chiang; Hsuan Hui Wang; You Cheng Hseu

doi:10.1016/j.fct.2006.04.006

Protection from oxidative damage using Bidens pilosa extracts in normal human erythrocytes

Hsin Ling Yang, Ssu Ching Chen, Nai Wen Chang, Jia Ming Chang, Mei Ling Lee, Pei Chuan Tsai, Han Hsuan Fu, Wei Wan Kao, Hsiao Chi Chiang, Hsuan Hui Wang, You Cheng Hseu

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

116 Scopus citations

Abstract

Bidens pilosa (B. pilosa) is well known in Taiwan as a traditional Chinese medicine. The purpose of this study was to evaluate the ability of both the ethanol (EtOH) and ethylacetate/ethanol (EA/EtOH) extracts from the whole B. pilosa plant, to protect normal human erythrocytes against oxidative damage in vitro. It was determined that the oxidative hemolysis and lipid/protein peroxidation of erythrocytes induced by the aqueous peroxyl radical [2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)] were suppressed by both EtOH (50-150 μg/ml) and EA/EtOH (25-75 μg/ml) extracts of B. pilosa in concentration- and time-dependent manners. B. pilosa extracts also prevented the decline of superoxide dismutase (SOD) activity and the depletion of cytosolic glutathione (GSH) and ATP in erythrocytes. These results imply that B. pilosa may have protective antioxidant properties.

Original language	English
Pages (from-to)	1513-1521
Number of pages	9
Journal	Food and Chemical Toxicology
Volume	44
Issue number	9
DOIs	https://doi.org/10.1016/j.fct.2006.04.006
State	Published - Sep 2006

Keywords

Antioxidants
Bidens pilosa
Glutathione
Hemolysis
Superoxide dismutase

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.fct.2006.04.006

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title = "Protection from oxidative damage using Bidens pilosa extracts in normal human erythrocytes",

abstract = "Bidens pilosa (B. pilosa) is well known in Taiwan as a traditional Chinese medicine. The purpose of this study was to evaluate the ability of both the ethanol (EtOH) and ethylacetate/ethanol (EA/EtOH) extracts from the whole B. pilosa plant, to protect normal human erythrocytes against oxidative damage in vitro. It was determined that the oxidative hemolysis and lipid/protein peroxidation of erythrocytes induced by the aqueous peroxyl radical [2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)] were suppressed by both EtOH (50-150 μg/ml) and EA/EtOH (25-75 μg/ml) extracts of B. pilosa in concentration- and time-dependent manners. B. pilosa extracts also prevented the decline of superoxide dismutase (SOD) activity and the depletion of cytosolic glutathione (GSH) and ATP in erythrocytes. These results imply that B. pilosa may have protective antioxidant properties.",

keywords = "Antioxidants, Bidens pilosa, Glutathione, Hemolysis, Superoxide dismutase",

author = "Yang, {Hsin Ling} and Chen, {Ssu Ching} and Chang, {Nai Wen} and Chang, {Jia Ming} and Lee, {Mei Ling} and Tsai, {Pei Chuan} and Fu, {Han Hsuan} and Kao, {Wei Wan} and Chiang, {Hsiao Chi} and Wang, {Hsuan Hui} and Hseu, {You Cheng}",

note = "Funding Information: This work was supported by grants NSC 92-2815-C-039-003-B and NSC 92-2320-B-039-047 from the National Science of the Republic of China. Human umbilical vein endothelial cells (HUVECs) were kindly provided by the Department of Obstetrics and Gynecology, China Medical University Hospital.",

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T1 - Protection from oxidative damage using Bidens pilosa extracts in normal human erythrocytes

AU - Yang, Hsin Ling

AU - Chen, Ssu Ching

AU - Chang, Nai Wen

AU - Chang, Jia Ming

AU - Lee, Mei Ling

AU - Tsai, Pei Chuan

AU - Fu, Han Hsuan

AU - Kao, Wei Wan

AU - Chiang, Hsiao Chi

AU - Wang, Hsuan Hui

AU - Hseu, You Cheng

N1 - Funding Information: This work was supported by grants NSC 92-2815-C-039-003-B and NSC 92-2320-B-039-047 from the National Science of the Republic of China. Human umbilical vein endothelial cells (HUVECs) were kindly provided by the Department of Obstetrics and Gynecology, China Medical University Hospital.

PY - 2006/9

Y1 - 2006/9

N2 - Bidens pilosa (B. pilosa) is well known in Taiwan as a traditional Chinese medicine. The purpose of this study was to evaluate the ability of both the ethanol (EtOH) and ethylacetate/ethanol (EA/EtOH) extracts from the whole B. pilosa plant, to protect normal human erythrocytes against oxidative damage in vitro. It was determined that the oxidative hemolysis and lipid/protein peroxidation of erythrocytes induced by the aqueous peroxyl radical [2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)] were suppressed by both EtOH (50-150 μg/ml) and EA/EtOH (25-75 μg/ml) extracts of B. pilosa in concentration- and time-dependent manners. B. pilosa extracts also prevented the decline of superoxide dismutase (SOD) activity and the depletion of cytosolic glutathione (GSH) and ATP in erythrocytes. These results imply that B. pilosa may have protective antioxidant properties.

AB - Bidens pilosa (B. pilosa) is well known in Taiwan as a traditional Chinese medicine. The purpose of this study was to evaluate the ability of both the ethanol (EtOH) and ethylacetate/ethanol (EA/EtOH) extracts from the whole B. pilosa plant, to protect normal human erythrocytes against oxidative damage in vitro. It was determined that the oxidative hemolysis and lipid/protein peroxidation of erythrocytes induced by the aqueous peroxyl radical [2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)] were suppressed by both EtOH (50-150 μg/ml) and EA/EtOH (25-75 μg/ml) extracts of B. pilosa in concentration- and time-dependent manners. B. pilosa extracts also prevented the decline of superoxide dismutase (SOD) activity and the depletion of cytosolic glutathione (GSH) and ATP in erythrocytes. These results imply that B. pilosa may have protective antioxidant properties.

KW - Antioxidants

KW - Bidens pilosa

KW - Glutathione

KW - Hemolysis

KW - Superoxide dismutase

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U2 - 10.1016/j.fct.2006.04.006

DO - 10.1016/j.fct.2006.04.006

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JO - Food and Chemical Toxicology

JF - Food and Chemical Toxicology

IS - 9

ER -

Protection from oxidative damage using Bidens pilosa extracts in normal human erythrocytes

Abstract

Keywords

UN SDGs

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