Production of interferon-β by NB1-RGB cells cultured on peptide-lipid membranes

Mariko Hara; Yasunari Takanashi; Nobuya Tuzuki; Hiroko Kawakami; Kazunori Toma; Akon Higuchi

doi:10.1023/A:1026157515032

Production of interferon-β by NB1-RGB cells cultured on peptide-lipid membranes

Mariko Hara, Yasunari Takanashi, Nobuya Tuzuki, Hiroko Kawakami, Kazunori Toma, Akon Higuchi

Department of Chemical and Materials Engineering

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Cell growth and production of interferon-β (IFN-β) were investigated for normal human skin fibroblast cells (NB1-RGB) cultured on membranes prepared from peptide-lipids containing the arginine-glycine-aspartic acid [Arg-Gly-Asp] (RGD), tyrosine-isoleucine-glycine-serine-arginine [Tyr-Ile-Gly-Ser-Arg] (YIGSR) and arginine-glutamic acid-aspartic acid-valine [Arg-Glu-Asp-Val] (REDV) peptides. Cell density was found to be approximately the same on various peptide-lipid membranes, whereas production of IFN-β depended significantly on the peptide-lipid membranes on which NB1-RGB cells were cultured. The highest production of IFN-β was observed for NB1-RGB cells on REDV-lipid membranes prepared by a casting method (REDV-cast membranes) after 24 hr of cultivation. Specific binding between REDV of REDV-cast membranes and the receptor on the NB1-RGB cells may have caused the specific cell response for the production of IFN-β.

Original language	English
Pages (from-to)	13-20
Number of pages	8
Journal	Cytotechnology
Volume	42
Issue number	1
DOIs	https://doi.org/10.1023/A:1026157515032
State	Published - 2003

Keywords

Cell culture
Fibroblast cells
Interferon-β
Langmuir-Blodgett membrane
Peptide-lipid

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title = "Production of interferon-β by NB1-RGB cells cultured on peptide-lipid membranes",

abstract = "Cell growth and production of interferon-β (IFN-β) were investigated for normal human skin fibroblast cells (NB1-RGB) cultured on membranes prepared from peptide-lipids containing the arginine-glycine-aspartic acid [Arg-Gly-Asp] (RGD), tyrosine-isoleucine-glycine-serine-arginine [Tyr-Ile-Gly-Ser-Arg] (YIGSR) and arginine-glutamic acid-aspartic acid-valine [Arg-Glu-Asp-Val] (REDV) peptides. Cell density was found to be approximately the same on various peptide-lipid membranes, whereas production of IFN-β depended significantly on the peptide-lipid membranes on which NB1-RGB cells were cultured. The highest production of IFN-β was observed for NB1-RGB cells on REDV-lipid membranes prepared by a casting method (REDV-cast membranes) after 24 hr of cultivation. Specific binding between REDV of REDV-cast membranes and the receptor on the NB1-RGB cells may have caused the specific cell response for the production of IFN-β.",

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T1 - Production of interferon-β by NB1-RGB cells cultured on peptide-lipid membranes

AU - Hara, Mariko

AU - Takanashi, Yasunari

AU - Tuzuki, Nobuya

AU - Kawakami, Hiroko

AU - Toma, Kazunori

AU - Higuchi, Akon

PY - 2003

Y1 - 2003

N2 - Cell growth and production of interferon-β (IFN-β) were investigated for normal human skin fibroblast cells (NB1-RGB) cultured on membranes prepared from peptide-lipids containing the arginine-glycine-aspartic acid [Arg-Gly-Asp] (RGD), tyrosine-isoleucine-glycine-serine-arginine [Tyr-Ile-Gly-Ser-Arg] (YIGSR) and arginine-glutamic acid-aspartic acid-valine [Arg-Glu-Asp-Val] (REDV) peptides. Cell density was found to be approximately the same on various peptide-lipid membranes, whereas production of IFN-β depended significantly on the peptide-lipid membranes on which NB1-RGB cells were cultured. The highest production of IFN-β was observed for NB1-RGB cells on REDV-lipid membranes prepared by a casting method (REDV-cast membranes) after 24 hr of cultivation. Specific binding between REDV of REDV-cast membranes and the receptor on the NB1-RGB cells may have caused the specific cell response for the production of IFN-β.

AB - Cell growth and production of interferon-β (IFN-β) were investigated for normal human skin fibroblast cells (NB1-RGB) cultured on membranes prepared from peptide-lipids containing the arginine-glycine-aspartic acid [Arg-Gly-Asp] (RGD), tyrosine-isoleucine-glycine-serine-arginine [Tyr-Ile-Gly-Ser-Arg] (YIGSR) and arginine-glutamic acid-aspartic acid-valine [Arg-Glu-Asp-Val] (REDV) peptides. Cell density was found to be approximately the same on various peptide-lipid membranes, whereas production of IFN-β depended significantly on the peptide-lipid membranes on which NB1-RGB cells were cultured. The highest production of IFN-β was observed for NB1-RGB cells on REDV-lipid membranes prepared by a casting method (REDV-cast membranes) after 24 hr of cultivation. Specific binding between REDV of REDV-cast membranes and the receptor on the NB1-RGB cells may have caused the specific cell response for the production of IFN-β.

KW - Cell culture

KW - Fibroblast cells

KW - Interferon-β

KW - Langmuir-Blodgett membrane

KW - Peptide-lipid

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DO - 10.1023/A:1026157515032

M3 - 期刊論文

C2 - 19002924

AN - SCOPUS:0242642144

SN - 0920-9069

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SP - 13

EP - 20

JO - Cytotechnology

JF - Cytotechnology

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Production of interferon-β by NB1-RGB cells cultured on peptide-lipid membranes

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Keywords

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