Probiotic activity of staphylococcus epidermidis induces collagen type i production through FFAR2/P-ERK signaling

Indira Putri Negari, Sunita Keshari, Chun Ming Huang

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Collagen type I is a key structural component of dermis tissue and is produced by fi-broblasts and the extracellular matrix. The skin aging process, which is caused by intrinsic or extrinsic factors, such as natural aging or free radical exposure, greatly reduces collagen expression, thereby leading to obstructed skin elasticity. We investigated the effective fermentation of Cetearyl isononanoate (CIN), a polyethylene glycol (PEG) analog, as a carbon source with the skin probiotic bacterium Staphylococcus epidermidis (S. epidermidis) or butyrate, as their fermentation metabolites could noticeably restore collagen expression through phosphorylated extracellular signal regulated kinase (p-ERK) activation in mouse fibroblast cells and skin. Both the in vitro and in vivo knockdown of short-chain fatty acid (SCFA) or free fatty acid receptor 2 (FFaR2) considerably blocked the probi-otic effect of S. epidermidis on p-ERK-induced collagen type I induction. These results demonstrate that butyric acid (BA) in the metabolites of fermenting skin probiotic bacteria mediates FFaR2 to induce the synthesis of collagen through p-ERK activation. We hereby imply that metabolites from the probiotic S. epidermidis fermentation of CIN as a potential carbon source could restore impaired collagen in the dermal extracellular matrix (ECM), providing integrity and elasticity to skin.

Original languageEnglish
Article number1414
Pages (from-to)1-14
Number of pages14
JournalInternational Journal of Molecular Sciences
Issue number3
StatePublished - 2 Feb 2021


  • Butyric acid
  • Collagen type I
  • Probiotic
  • Staphylococcus epidermidis


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