Probing bacterial cell wall growth by tracing wall-anchored protein complexes

Yi Jen Sun, Fan Bai, An Chi Luo, Xiang Yu Zhuang, Tsai Shun Lin, Yu Cheng Sung, Yu Ling Shih, Chien Jung Lo

Research output: Contribution to journalArticlepeer-review

Abstract

The dynamic assembly of the cell wall is key to the maintenance of cell shape during bacterial growth. Here, we present a method for the analysis of Escherichia coli cell wall growth at high spatial and temporal resolution, which is achieved by tracing the movement of fluorescently labeled cell wall-anchored flagellar motors. Using this method, we clearly identify the active and inert zones of cell wall growth during bacterial elongation. Within the active zone, the insertion of newly synthesized peptidoglycan occurs homogeneously in the axial direction without twisting of the cell body. Based on the measured parameters, we formulate a Bernoulli shift map model to predict the partitioning of cell wall-anchored proteins following cell division.

Original languageEnglish
Article number2160
JournalNature Communications
Volume12
Issue number1
DOIs
StatePublished - Dec 2021

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