Preparation of temperature-responsive Pluronic immobilized membrane and storage of hematopoietic stem cells in culture flask

Satsuki Egashira, Yumiko Gomei, Taro Yamamoto, Nobuo Aoki, Sizue Natori, Akon Higuchi

Research output: Contribution to conferencePaperpeer-review

Abstract

The bio-inert materials on which cells do not proliferate, differentiate nor de-differentiate should be useful for the culture and preservation of stem cells. The Pluronic F127 and F68, a triblock copolymer of ethylene oxide, and propylene oxide were activated using carbonyldiimidazole (CDI), and CDI-activated Pluronic was subsequently immobilized on the surface of a lysine-coated polystyrene tissue culture flask and culture bag. The morphology of fibroblasts on the Pluronic-immobilized flask was spherical, and did not show spreading behavior. This observation indicates that L929 cells on Pluronic-immobilized flasks were cultured in a bio-inert environment. The expression ratio of surface markers on hematopoietic stem cells (CD34) cultured in the Pluronic-immobilized flask and bag were significantly higher than nonmodified culture flask. This is caused by the existence of flexible brush-like segments of Pluronic on the Pluronic-immobilized flask and bag.

Original languageEnglish
Pages1995
Number of pages1
StatePublished - 2006
Event55th SPSJ Annual Meeting - Nagoya, Japan
Duration: 24 May 200626 May 2006

Conference

Conference55th SPSJ Annual Meeting
Country/TerritoryJapan
CityNagoya
Period24/05/0626/05/06

Keywords

  • Biocompatibility
  • Hematopoietic stem cell
  • Pluronic

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