Abstract
The bio-inert materials on which cells do not proliferate, differentiate nor de-differentiate should be useful for the culture and preservation of stem cells. The Pluronic F127 and F68 were activated using carbonyldiimidazole (CDI), and CDI-activated Pluronic was subsequently immobilized on the surface of a lysine-coated polystyrene tissue culture flask. The morphology of fibroblasts (L929 cells) on the Pluronic-immobilized flask was spherical, and did not show spreading behavior. This observation indicates that L929 cells on Pluronic-immobilized flasks were cultured in a bio-inert environment. The expression ratio of surface markers on hematopoietic stem cells (CD34 and CD133) cultured in the Pluronic-immobilized flask was significantly higher than PSt tissue culture flask. This is caused by the existence of flexible brush-like segments of Pluronic on the Pluronic-immobilized flask.
Original language | English |
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Pages | 1921 |
Number of pages | 1 |
State | Published - 2006 |
Event | 55th SPSJ Annual Meeting - Nagoya, Japan Duration: 24 May 2006 → 26 May 2006 |
Conference
Conference | 55th SPSJ Annual Meeting |
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Country/Territory | Japan |
City | Nagoya |
Period | 24/05/06 → 26/05/06 |
Keywords
- Bio-inert
- Cell detachment
- Stem Cell
- Temperature Responsive Polymer