Preparation and cell culture of pluronic-immobilized cell culture flask

Nobuo Aoki; Taro Yamamoto; Yumiko Gomei; Satsuki Egashira; Shizue Natori; Akon Higuchi

Preparation and cell culture of pluronic-immobilized cell culture flask

Nobuo Aoki, Taro Yamamoto, Yumiko Gomei, Satsuki Egashira, Shizue Natori, Akon Higuchi

Department of Chemical and Materials Engineering

Research output: Contribution to conference › Paper › peer-review

Abstract

The bio-inert materials on which cells do not proliferate, differentiate nor de-differentiate should be useful for the culture and preservation of stem cells. The Pluronic F127 and F68 were activated using carbonyldiimidazole (CDI), and CDI-activated Pluronic was subsequently immobilized on the surface of a lysine-coated polystyrene tissue culture flask. The morphology of fibroblasts (L929 cells) on the Pluronic-immobilized flask was spherical, and did not show spreading behavior. This observation indicates that L929 cells on Pluronic-immobilized flasks were cultured in a bio-inert environment. The expression ratio of surface markers on hematopoietic stem cells (CD34 and CD133) cultured in the Pluronic-immobilized flask was significantly higher than PSt tissue culture flask. This is caused by the existence of flexible brush-like segments of Pluronic on the Pluronic-immobilized flask.

Original language	English
Pages	1921
Number of pages	1
State	Published - 2006
Event	55th SPSJ Annual Meeting - Nagoya, Japan Duration: 24 May 2006 → 26 May 2006

Conference

Conference	55th SPSJ Annual Meeting
Country/Territory	Japan
City	Nagoya
Period	24/05/06 → 26/05/06

Keywords

Bio-inert
Cell detachment
Stem Cell
Temperature Responsive Polymer

Cite this

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title = "Preparation and cell culture of pluronic-immobilized cell culture flask",

abstract = "The bio-inert materials on which cells do not proliferate, differentiate nor de-differentiate should be useful for the culture and preservation of stem cells. The Pluronic F127 and F68 were activated using carbonyldiimidazole (CDI), and CDI-activated Pluronic was subsequently immobilized on the surface of a lysine-coated polystyrene tissue culture flask. The morphology of fibroblasts (L929 cells) on the Pluronic-immobilized flask was spherical, and did not show spreading behavior. This observation indicates that L929 cells on Pluronic-immobilized flasks were cultured in a bio-inert environment. The expression ratio of surface markers on hematopoietic stem cells (CD34 and CD133) cultured in the Pluronic-immobilized flask was significantly higher than PSt tissue culture flask. This is caused by the existence of flexible brush-like segments of Pluronic on the Pluronic-immobilized flask.",

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TY - CONF

T1 - Preparation and cell culture of pluronic-immobilized cell culture flask

AU - Aoki, Nobuo

AU - Yamamoto, Taro

AU - Gomei, Yumiko

AU - Egashira, Satsuki

AU - Natori, Shizue

AU - Higuchi, Akon

PY - 2006

Y1 - 2006

N2 - The bio-inert materials on which cells do not proliferate, differentiate nor de-differentiate should be useful for the culture and preservation of stem cells. The Pluronic F127 and F68 were activated using carbonyldiimidazole (CDI), and CDI-activated Pluronic was subsequently immobilized on the surface of a lysine-coated polystyrene tissue culture flask. The morphology of fibroblasts (L929 cells) on the Pluronic-immobilized flask was spherical, and did not show spreading behavior. This observation indicates that L929 cells on Pluronic-immobilized flasks were cultured in a bio-inert environment. The expression ratio of surface markers on hematopoietic stem cells (CD34 and CD133) cultured in the Pluronic-immobilized flask was significantly higher than PSt tissue culture flask. This is caused by the existence of flexible brush-like segments of Pluronic on the Pluronic-immobilized flask.

AB - The bio-inert materials on which cells do not proliferate, differentiate nor de-differentiate should be useful for the culture and preservation of stem cells. The Pluronic F127 and F68 were activated using carbonyldiimidazole (CDI), and CDI-activated Pluronic was subsequently immobilized on the surface of a lysine-coated polystyrene tissue culture flask. The morphology of fibroblasts (L929 cells) on the Pluronic-immobilized flask was spherical, and did not show spreading behavior. This observation indicates that L929 cells on Pluronic-immobilized flasks were cultured in a bio-inert environment. The expression ratio of surface markers on hematopoietic stem cells (CD34 and CD133) cultured in the Pluronic-immobilized flask was significantly higher than PSt tissue culture flask. This is caused by the existence of flexible brush-like segments of Pluronic on the Pluronic-immobilized flask.

KW - Bio-inert

KW - Cell detachment

KW - Stem Cell

KW - Temperature Responsive Polymer

UR - http://www.scopus.com/inward/record.url?scp=33749856923&partnerID=8YFLogxK

M3 - 會議論文

AN - SCOPUS:33749856923

SP - 1921

Y2 - 24 May 2006 through 26 May 2006

ER -

Preparation and cell culture of pluronic-immobilized cell culture flask

Abstract

Conference

Keywords

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