Potentiometric quantitation of glycerol using immobilized glycerol dehydrogenase

A. K. Chen; J. A. Starzmann; C. C. Liu

doi:10.1002/bit.260240416

Potentiometric quantitation of glycerol using immobilized glycerol dehydrogenase

A. K. Chen, J. A. Starzmann, C. C. Liu

Graduate Institute of Materials Science and Engineering

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Glycerol dehydrogenase was immobilized in polyacrylamide gel layered over a small platinum screen and used to catalyze the oxidation of glycerol. In the presence of NAD⁺ and potassium ferricyanide, the coupling reaction generated a measurable electrical potential which was found to be Nernstian with respect to the glycerol concentration range of 10⁻⁴M to 10⁻¹M. The reproducibility of the measurement and the optimal conditions for glycerol determination were described.

Original language	English
Pages (from-to)	971-975
Number of pages	5
Journal	Biotechnology and Bioengineering
Volume	24
Issue number	4
DOIs	https://doi.org/10.1002/bit.260240416
State	Published - Apr 1982

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10.1002/bit.260240416

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title = "Potentiometric quantitation of glycerol using immobilized glycerol dehydrogenase",

abstract = "Glycerol dehydrogenase was immobilized in polyacrylamide gel layered over a small platinum screen and used to catalyze the oxidation of glycerol. In the presence of NAD+ and potassium ferricyanide, the coupling reaction generated a measurable electrical potential which was found to be Nernstian with respect to the glycerol concentration range of 10−4M to 10−1M. The reproducibility of the measurement and the optimal conditions for glycerol determination were described.",

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AU - Chen, A. K.

AU - Starzmann, J. A.

AU - Liu, C. C.

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Y1 - 1982/4

N2 - Glycerol dehydrogenase was immobilized in polyacrylamide gel layered over a small platinum screen and used to catalyze the oxidation of glycerol. In the presence of NAD+ and potassium ferricyanide, the coupling reaction generated a measurable electrical potential which was found to be Nernstian with respect to the glycerol concentration range of 10−4M to 10−1M. The reproducibility of the measurement and the optimal conditions for glycerol determination were described.

AB - Glycerol dehydrogenase was immobilized in polyacrylamide gel layered over a small platinum screen and used to catalyze the oxidation of glycerol. In the presence of NAD+ and potassium ferricyanide, the coupling reaction generated a measurable electrical potential which was found to be Nernstian with respect to the glycerol concentration range of 10−4M to 10−1M. The reproducibility of the measurement and the optimal conditions for glycerol determination were described.

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DO - 10.1002/bit.260240416

M3 - 期刊論文

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VL - 24

SP - 971

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JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

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ER -

Potentiometric quantitation of glycerol using immobilized glycerol dehydrogenase

Abstract

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