Potentiometric quantitation of glycerol using immobilized glycerol dehydrogenase

A. K. Chen, J. A. Starzmann, C. C. Liu

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


Glycerol dehydrogenase was immobilized in polyacrylamide gel layered over a small platinum screen and used to catalyze the oxidation of glycerol. In the presence of NAD+ and potassium ferricyanide, the coupling reaction generated a measurable electrical potential which was found to be Nernstian with respect to the glycerol concentration range of 10−4M to 10−1M. The reproducibility of the measurement and the optimal conditions for glycerol determination were described.

Original languageEnglish
Pages (from-to)971-975
Number of pages5
JournalBiotechnology and Bioengineering
Issue number4
StatePublished - Apr 1982


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