Potentiometric method for subatrate analysis using immobilized NAD+‐dependent oxidoreductase enzymes

A. K. Chen, C. C. Liu, J. G. Schiller

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Two coenzyme‐dependent oxidoreductases, glucose dehydrogenase and alcohol dehydrogenase, were immobilized in polyacrylamide gel over a platinum grid matrix and used as enzyme electrodes to measure their substrate concentrations in buffered aqueous solutions. The immobilized enzymes were used to oxidize their substrates in the presence of NAD+. Ferricyanide was used as the redox mediator and electroactive specific. The determinations of glucose and ethenol were utilized to demonstrate and evaluate the performance of the system. The described methodology should be readily applicable to the analysis of numerous other substrates of coenzyme‐dependent oxidoreductases.

Original languageEnglish
Pages (from-to)1905-1915
Number of pages11
JournalBiotechnology and Bioengineering
Volume21
Issue number11
DOIs
StatePublished - Nov 1979

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