Para-phenylenediamine induced DNA damage and apoptosis through oxidative stress and enhanced caspase-8 and -9 activities in Mardin-Darby canine kidney cells

S. C. Chen, C. H. Chen, Y. L. Tioh, P. Y. Zhong, Y. S. Lin, S. M. Chye

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Para-phenylenediamine (p-PD), a suspected carcinogen, is a component of permanent hair dyes. In this study we examined the mechanism of cytotoxicity and genotoxicity in Mardin-Darby canine kidney cells (MDCK)-treated with p-PD. Our results showed that p-PD decreased cell viability in a dose- and time-dependent manner. In addition, p-PD induced DNA damage was confirmed by the comet and TUNEL assays. Pre-treatment of MDCK cells with antioxidants vitamin C or E significantly inhibited p-PD induced cytotoxicity and reactive oxygen species (ROS) generation. Furthermore, p-PD induced apoptosis through activated initiator caspase-8 and -9, and effector caspase-3/7. Based on these results, we suggested that p-PD induce apoptosis which was mediated with caspase-8, caspase-9 and caspase-3/7 activation via the involvement of ROS.

Original languageEnglish
Pages (from-to)1197-1202
Number of pages6
JournalToxicology in Vitro
Volume24
Issue number4
DOIs
StatePublished - Jun 2010

Keywords

  • Caspase
  • DNA damage
  • Mardin-Darby canine kidney cells
  • Para-Phenylenediamine
  • Reactive oxygen species

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